| 1. | Subcloning of 32kda proteins gene of schistosoma japonicum in the eukaryocyte expression vector
蛋白质基因在真核表达载体中的亚克隆 |
| 2. | Plant expression vector pespcema was constructed after subcloning in puc121 and other vectors
经puc121等中间载体的亚克隆，构建了pespcema植物表达载体。 |
| 3. | With a series of hybridization and subcloning , the two deletion end - points of this deleted region and deletion junction were localized precisely and cloned
通过southern杂交和亚克隆，精确定位和克隆了这段缺失区域的两个端点及缺失界点。 |
| 4. | One solobp ecor i fragment containing phospholipase gene was isolated . further sequence analysis and subcloning revealed a 963bp phla . gene coding a 320aa phospholipase phl with deduced molecular weight of 33kd
通过测序及亚克隆分析，发现一个磷酯酶的基因phla ，长度为963bp ，预测编码一个由320个氨基酸组成，分子量为33kd的磷酯酶phl 。 |
| 5. | As heterologous probe and subsequently show to code for desired enzymatic activity . after a serial of subcloning coupled with southern hybridization and enzymatic activity assay , the functional s . griseus atcc14811 cholesterol oxidase gene ( chog ) was localized onto 2 . 3kb ecori - sall fragment
对phz1140和phz1141进行bamh及bgl的酶谱分析及与choa探针的杂交，将胆同醇氧化酶基因初步定位在8 . 8kbbamh和9 . 9kbbgl片段上。 |
| 6. | Sequence analysis showed that , this fragment has a homology of 99 % to the previously reported choe from rhodococcus equi . after cloning and subcloning and three identical fragments were obtained from three independent pcr , lacking three continual bases ( ttc , encoding the pheamino acid ) compared with choe , thus it can be assumed that the new fragment , designated as choew , and choe belong to the same gene family , even it might be the natural mutation of choe
对片段进行克隆、亚克隆之后测序分析，发现与已克隆的来源于马红球菌的胆固醇氧化酶基因cboe有99的同源性；并且三次独立的pcr均得到相同的片段，所克隆的基因序列与choe相比，连续缺失三个碱基( ttc ) ，即相应的氨基酸序列缺失一个氨基酸( phe ) ，因此判断所克隆的基因片段与choe属同一基因家族或原有基因的天然突变体，命名为choew 。 |
| 7. | The insert dna fragments are 7kb and llkb , respectively . two subclones that were designated pgr3h1 and pgr7h1 and can increase glyphosate resistance of e . coli jm109 up to 150mm glyphosate were constructed by subcloning the 2 . 4kb and 3 . 2 kb hind ? / psti fragments of pgr3 and pgr7 into the corresponding sites of pgem - 3zf and pbluescript . sequence analysis of these two subclones revealed a completely identical 1323bp open reading frame that encodes an epsp synthase
以pgem - 3zf和pbluescript为载体，利用限制性内切酶hind和pst构建这两个克隆的亚克隆，从中分别得到两个草甘膦耐受亚克隆pgr3h1和pgr7h1 ，插入片段各为2 . 4kb和3 . 2kb ，对这两个亚克隆进行序列分析，发现二者均含有一个核苷酸序列完全相同的完整的epsp合成酶基因? ? aroa ，其核苷酸序列长为1323bp ，推导的epsp合成酶由441个氨基酸组成，两个亚克隆的草甘膦耐受浓度最大可达150mm 。 |
| 8. | The bvdv nadl strain is cytopathogenic . searching fromgenbank several cp strains " sequence of e2 gene about deer - n21 , sh9 , newyork - i and so on were found . according to the homologous sequence they were designed and synthesised a pair of primers by the biology software primer 5 and added bal i and nco i site to the 5 " end which can be used for the application of e2 gene and subcloning
从genbank中搜寻出deer - n _ ( 21 ) 、 sh9 、 newyork -等cp型毒株的e _ 2基因序列，根据其同源性用生物学软件primer5设计引物，并在引物的两端加入bal和nco两个酶切位点，酶切位点的存在易于对e _ 2基因进行克隆操作。 |
| 9. | Using enterobacter cloacae b8 , the mutated strains b8b and b8f , and the recombinant clones pb and pf , we try to sequence the antagonistic - related genes of enterobacter cloacae b8 by subcloning and genome primering system . the acquired sequences were analyzed with blast program to find any homology to sequences deposited in genebank
以广谱拮抗菌阴沟肠杆菌b8菌株和拮抗活性缺失菌株b8b 、 b8f及从b8b和b8f二菌株克隆获得的重组质粒pb 、 pf为基础，对阴沟肠杆菌b8菌株拮抗相关的b和f基因片段进行序列分析。 |
