| 1. | No experimental animals of different breeds, strains or sublines may be mixed together in transportation
不得将不同品种、品系或者不同等级的实验动物混合装运。 |
| 2. | Recent developments in molecular biotechnology are making this possible . in the work described here, three transgenic sublines of embryogenic norway spruce calli : a78-3, a78-4 and a78-5 were studied
随着分子生物学技术的发展，通过转基因技术有目的地培育出木质素含量低的挪威云杉新品种就成为可能。 |
| 3. | Furthermore the expressions of all the genes in a representative sample were examed by the recently developed method of hybridization to cdna arrays . this was intended to strengthen the theoretical background for the screening of norway spruce genotypes with low lignin content . the calli of the transformed sublines a78-3, a78-4, a78-5 and the untransformed control a95 : 88 : 22 were successfully induced to form mature embryos from which plantlets were established
以转基因亚系a78-3、a78-4、a78-5和未转基因对照a95:88:22的细胞愈伤组织为实验材料，以dkm和lp-m熟化培养基培养五周后，再以14sh萌发培养基培养四周，成功地诱导形成了胚胎，并再生成新的小植株，萌发成活率达到80。 |
| 4. | They contained an antisense constructed for the spruce gene encoding ccr ( cinnamoyl alcohol dehydrogenase ), an enzyme of monolignol synthesis . the antisense rna method is a technique for reducing the expression of a resident target gene . the transgenic sublines were produced by particle bombardment at the dept of forest genetics, swedish university of agricultural sciences
本项目研究的目的就是采用瑞典农业大学构建的反义ccr基因转导的挪威云杉细胞愈伤组织，通过诱导产生再生植株，并检测证实该基因是否表达及其它相关基因的表达状况，为培育出低木质素的转基因挪威云杉新品种奠定物质和理论基础。 |
| 5. | These indicated that the expressed difference of a78-3 and a78-4 were much higher than that of a78-5; ( 2 ) in comparison to the control line, 0.26 %-2.60 % of the total genes in the array expressed significant differences in sublines a78-3 and a78-4, but not in a78-5 . these also confirmed the rt-pcr result once again and revealed some related genes have been affected by the expression of the antisense ccr
这说明在再生小植株这个发育阶段由于很多器官的分化所造成的在外部形态上的差异来源于其内部基因的表达的差异；（4）表达呈显著差异的基因按其功能归类，发现在与对照比较时，在a783和a784两个亚系表达呈显著差异的14个基因中，第1大类与苯丙氨酸循环有关的基因表达差异比例最高，占芯片同类基因的8 |
| 6. | In contrast, there was very little difference in gene expression between calli subcultured for one week and two weeks with only 0.78 % of the genes showing significant different expression . ( 4 ) the 14 genes with different expression level between the control and the transformed sublines a78-3 and a78-4 were classified by their function
88的未鉴定功能基因的表达呈显著差异；而在愈伤组织培养二周与愈伤组织培养一周之间，则在第大类、第v大类和第大类未鉴定基因中各有1个表达呈显著差异。 |
| 7. | The results shows that germination with 1 / 4 strength sh medium after maturation for 5 weeks with dkm or lp-m maturing medium ( containing aba ) is a suitable combination for these sublines since the viability reached 80 % . total rna was isolated from the calli and plantlets of each transgenic subline and respective controls . rt-pcr results showed that the presence of the ccr antisense band in sublines a78-3 and a78-4 but not in a78-5 or the untransformed control a95 : 88 : 22
结果显示：在愈伤组织和再生小植株中，a78-3和a78-4两个亚系都有很明显的反义带，但a78-5没有出现反义带，从而证实了a78-3和a78-4两个亚系的反义ccr基因已经在愈伤组织和再生小植株中都得到了成功的整合和表达，而a78-5则没有表达。 |
| 8. | The results shows that germination with 1 / 4 strength sh medium after maturation for 5 weeks with dkm or lp-m maturing medium ( containing aba ) is a suitable combination for these sublines since the viability reached 80 % . total rna was isolated from the calli and plantlets of each transgenic subline and respective controls . rt-pcr results showed that the presence of the ccr antisense band in sublines a78-3 and a78-4 but not in a78-5 or the untransformed control a95 : 88 : 22
结果显示：在愈伤组织和再生小植株中，a78-3和a78-4两个亚系都有很明显的反义带，但a78-5没有出现反义带，从而证实了a78-3和a78-4两个亚系的反义ccr基因已经在愈伤组织和再生小植株中都得到了成功的整合和表达，而a78-5则没有表达。 |
