Rules for forage seed testing biochemical topographical tetrazolium test for viability 生活力的生物化学四唑测定
4.
In this assay , a tetrazolium salt ( mtt ) is converted to a blue formazan product by enzymes active only in living cells . the amount of formazan produced can be quantitated using a microtitre plate reader Rta一yqrl蛋白的细胞毒性约为rra蛋白的2一40倍,提示高尔基体可能是毒素在细胞内转运过程中的重要一环。
5.
It was shown that vsmcs growth was suppressed by as2o3 with correlation to its concentration and the reaction time , especially marked in 72h with last concentration of 3 . 0 ~ 4 . 0umol / l as2o3 , by using 3 - ( 4 , 5 - dimethylthiazolzy ) - 2 , 5 - diphenyl tetrazolium bromide chromatometry analysis 首先应用四甲基偶氮唑蓝比色法分析显示as _ 2o _ 3能够抑制vsmc的生长,呈浓度及时间依赖性。在as _ 2o _ 3终浓度为3
6.
The biological activity of expressed products were examined with thiazoyl blue tetrazolium bromide ( mtt ) method , and the myoblast as target cell . it concluded that the biological activity of des ( l - 3 ) igf - l protein was 1 . 5 to 4 times higher than that of igf - 1 form 为了检测其生物活性,用重组人完整型igf - 1蛋白和截短型igf - 1蛋白分别刺激c2c12成肌细胞,然后用mtt (噻唑兰)法比较了这两种蛋白对c2c12成肌细胞的促增殖能力。