| 1. | The minimal amount of tgev cdna can be detected was about 120 pg in this research
本研究中探针tp检测到tgevcdna最低量为120pg 。 |
| 2. | In the research ts containing antigenic sites b and c was expressed with high efficiency
本研究高效表达了tgev含有b和c抗原位点的多肽片段( ts ) 。 |
| 3. | The 285 bp fragment ( named tp ) , locating at 5 ' terminus of spike gene , had high conservation in tgev isolations
Tgevs基因5 ’端285bp片段在tgev分离株中较为保守，与其它冠状病毒同源性较低。 |
| 4. | The anibodies to n protein of tgev could be detected in the serum of mice from group b at 39 days of pi
B组小鼠在首次免疫后第39d检测到针对tgevn蛋白的阳性抗体( p / n 2 . 0 ) ，其它时间均未检出阳性抗体。 |
| 5. | After the estimation of labeling efficiency tp was applicated to hybridize the rna of tgev , then was subjected to self - development of x - ray exposed by cspd to show the target nucleic acid
对标记效率测定后，以带正电的尼龙膜为介质进行特异性和灵敏性检测，经非放射性自显影来显示靶核酸。 |
| 6. | Spike protein was the main protective antigen inducing the neutralization antibody . the fragment containing antigenic sites b and c locating at the 5 " terminus in spike gene of tgev was deleted in prcv
Tgevs蛋白可诱导产生中和抗体，是主要的保护性抗原，其n端抗原位点b和c在prcv中缺失。 |
| 7. | Nonradioactive nucleic probe to tgev was prepared and was applicated to detect tgev at first stage , which established foundational work for differentiating tgev from prcv and investigation on epidemiology of tgev
制备了tgev非放射性标记的核酸探针，并初步应用于检测tgev 。二者为tgev鉴别诊断、血清学调查奠定了基础。 |
| 8. | The genome structure of tgev , pedv and prcv had high homology and it was difficult to diagnose tgev by traditional means . two studies were carried out to differentiate the tgev from prcv and investigate the epidemiology of tge
Tgev与猪流行性腹泻病毒( pedv ) 、猪呼吸道冠状病毒( prcv )在临床症状上很相似，基因组结构上与tgev具有较高的同源性，传统的方法很难鉴别。 |
| 9. | Transmissible gastroenteritis ( tge ) was an enteric disease of pigs caused by transmissible gastroenteritis virus ( tgev ) , a member of coronaviridae family . tgev was characterized with high mortality and had spread all over the world
猪传染性胃肠炎病毒( transmissiblegastroenteritisvirus , tgev )是冠状病毒科、冠状病毒属成员，引起猪的传染性胃肠炎( transmissiblegastroenteritistge ) ，死亡率很高，呈世界范围分布，严重危害养猪业的发展。 |
| 10. | Porcine transmissible gastroenteristis is an importan contagious disease endangering the development of swine . in other to establish a rapid diagnosis method and provide effective immunogenic products , the nucleoprotein ( n ) gene of porcine transmissible gastroenteristis virus ( tgev ) was cloned . expressed and its expressed product was purified
为建立对猪传染性胃肠炎快速有效的诊断方法，并试图在预防上提供有效的免疫制剂，本论文首次在我国对猪传染性胃肠炎病毒核衣壳蛋白基因进行了克隆、鉴定、表达及重组核蛋白的纯化；并在细胞上对重组核衣壳蛋白抗体的中和效力进行了测定。 |
