Construction of an expression system of epstein - barr virus thymidine kinase tk 的克隆及表达载体的构建
2.
Mechanism of action : stavudine , a nucleoside analogue of thymidine , inhibits the replication of hiv in human cells in vitro 司他夫定是胸苷核苷类似物，可抑制hiv病毒在人体细胞内的复制。
3.
We have identified six conserved domains by multiple sequence alignments of the thymidine kinase proteins of the alphaherpesviruses 通过对疱疹病毒tk氨基酸进行多序列比较，我们获得了在prvtk氨基酸序列上的六个保守区间。
4.
We also provide single mono - deoxynucleotide , such as deoxyadenosine monophosphate ( damp ) , deoxycytidine monophosphate ( dcmp ) , thymidine monophosphate disodium ( tmpna2 ) , deoxyguanosine monophosphate disodium ( dgmpna2 ) 我们还提供脱氧腺苷酸,脱氧鸟苷酸二钠,胸苷酸二钠,脱氧胸苷酸等的单体
5.
5 - fu is a inhibitor for purine metabolism and able to suppress various animal tumors . it can suppress thymidine synthetase to affect dna synthesis . in addition , it can also affect rna 化疗药物: 5 - fu为嘧啶类抗代谢药，对多种动物肿瘤有抑制作用，可抑制胸腺嘧啶脱氧核甘合成镅，影响dna的生物合成，此外，还可以作用于rna 。
6.
2 - e4 and s2 is induced respectively by 8 - ag and 5 - brdu with different drug concentration to make them deficient in hypoxanthine - guanine phosphoribosyl transferase ( hgprt ) and in thymidine kinase ( tk ) respectively and renamed 2 - e4 - a and 82 - 6 . their antibodies " isotypes are tested by goad anti - mouse isotype regent 取驯化好并处于对数生长期的2 - e _ 4 - a和s _ 2 - b细胞，再常规融合和筛选，三次克隆化后得稳定分泌双特异性抗体的杂交-杂交瘤细胞株6株。
7.
The determination of human thymidine kinase ( htk ) in human serum , which is a key indicator of cancers can give information for the diagnosis and treatment of the malign diseases . the protein a layer was first self - assembled onto the gold electrode surfaces of quartz crystals , the monoclonal antibodies were then orientedly immobilized through the specific binding between the fc terminals of the antibodies and the self - assembled protein a . with this sensor , the affinity constant of antigen - antibody binding was estimated to be 1 . 85 106 l / mol according to the scatchard ’ s plotting method , which proved the high bioactivity of antibody . finally , an amplified piezoelectric immunosensor was designed to determine the htk in 实验中将蛋白a吸附于镀金压电石英晶体电极表面,用于定向固定htk单克隆抗体,成功研制了检测htk的压电石英晶体传感器,并基于标准scatchard绘图法,计算出免疫反应的亲和常数为1 . 85 106l / mol ,证明该单克隆抗体具有较高的免疫活性;同时基于酶催化沉淀技术,设计了的检测htk的质量放大压电石英晶体传感器,该传感器可在0 . 1 - 10ng范围内对htk进行定量检测,应用此传感器成功地对5种癌症病人血清中htk的浓度进行了测定,实验结果为癌症的临床诊断与治疗提供了参考。
8.
Two spider silk protein genes ( avfl and avf2 ) screened from the library are characterized in a lot of repetitive motifs , a high guanosines or cytidines content , a strong preference for adenosine or thymidine in the third position of a codon and rich residues of glycines or alanines in the proteins translated 试验结果，该文库容量为4 . 9 10 ~ 6 。从文库中筛选到avf1和avf2蛛丝蛋白新基因，具有典型重复序列多， g c含量高，密码子第三个碱基偏爱使用a t及编码蛋白中含有大量gly和ala残基等特点。
9.
Pseudorabies virus ( prv ) is the causative agent of aujeszky ' s disease , which results in significant losses in pig husbandry . thymidine kinase ( tk ) gene is one of the main virulent genes of prv , and it is essential to the propagation of prv in the nerve tissues , but dispensable for virus replication and infection in other tissues 伪狂犬病是由伪狂犬病病毒( pseudorabiesvirus , prv )引起的家畜和多种野生动物的一种以发热、奇痒、呼吸和神经系统疾病为特征的急性传染病，妊娠母猪可发生死胎和流产，是危害养猪业的一个重要传染病。
