Koshiyama m , fujii h , kinezaki m , et al . immunohistochemical expression of topoisomerase iialpha ( topo ilalpha ) and multidrug resistance - associated protein ( mrp ) , plus chemosensitivity testing , as chemotherapeutic indices of ovarian and endometrial carcinomas [ j ] . anticaneer res , 2001 , 21 ( 4b ) : 2925 屠重棋,袁淑兰,王艳萍,等.外周血淋巴细胞和骨肉瘤细胞体外化疗药敏相关性研究[ j ] .中国肿瘤临床, 2003 , 30 ( 3 ) : 194
2.
In the laboratory experiment part , human peripheral blood , cultured cells and icr mice were study objects . the changes of mitotic chromosome numbers were measured by human metaphase chromosome counts and statistic analyzed used x2 - test . the changes of meiotic chromosome numbers were measured by mice one - cell zygote chromosome counts and statistic analyzed usedx2 - test . the effects of low dose ionizing radiation on the expression of topoisomerase ii were measured by immunocytochemistry , western blot and rt - pcr 流行病学结果显示长期小剂量辐射接触与染色体不分离呈正相关，为进一步在细胞遗传学和分子生物学方面研究小剂量电离辐射与染色体不分离关系及其机制，本课题第二部分以外周血、培养细胞、 icr小鼠为研究对象，用外周血染色体计数和单细胞受精卵染色体计数的方法研究小剂量辐射和拓扑异构酶复旦大学2000级博士生学位论文11a抑制剂及其二者的协同效应对有丝分裂和减数分裂染色体不分离的影响，用免疫细胞化学染色、 westernblot 、 rt pcr等方法研究了电离辐射引起拓扑异构酶a表达变化。
3.
The expression of topoisomerase ii of cultured cells treated low dose ionizing radiation decreased and then returned as time went . and become outstanding as radiation dose and frequency were added . inhibitor of topoisomerase ii could cause chromosome non - dis juction in mitosis and meiosis . and the co - effect of inhibitor of topoisomerase ii and ion 05 ） ，具有随照射次数增加而增加的趋势；小剂量电离辐射可以引起拓扑异构酶a表达变化，随照射后时间延长先下降后回升，随照射剂量和次数的增加，变化更加明显；拓扑异构酶11a抑制剂可引起有丝分裂和减数分裂时染色体的不分离；拓扑异构酶a抑制剂和电高辐射的协同作用使染色体不分离更加明显。
4.
The orf1 proteins of all four chinese senv isolates had two motifs ( ftl and yxxk ) which were conserved in replication - associated proteins . in all four chinese senv isolates , the putative non - structure orf2 protein had a cav - like region . the putative orf3 protein had a serine - rich tract preceded by a cluster of basic amino acids ( r and k ) . database scaning suggested this region had high a percentage of homology with dna topoisomerase i protein of d . melanogaster , and might play an important role in the replication of such single - stranded dna viruses 所有这四个中国分离株，均保留了o即1中与复制有关的保守序列( ftl和yxxk ) :在推测是非结构蛋白的orfz中均含有cav样保守区w ‘ x7一h一x3一c一xl一c一xs一h ; orf3蛋白的碱性氨基酸(精氨酸和赖氨酸)簇后紧接着一个富含丝氨酸的区域，与d . molanogaster的dna拓扑异构酶i有一定的同源性，推测在单链dna的复制中起作用。
