Chinese translation for "tricine"

两性离子缓冲剂

Example Sentences:

	1.	Gene had been successfully transformed and tris - tricine - page revealed that ，将表达载体转化到酵母saccharomyces cerevisiae中， southern杂交证明
	2.	The peptides were extracted , and analyzed using an improved tricine sodium dodecyl sulfate polyacrylamide gel electrophoresis ( tricine - sds - page ) method 方法通过针刺感染大肠杆菌诱导家蝇各虫态产生抗菌肽，再经研磨、离心等步骤提取这些抗菌肽，用改进的聚丙烯酰胺凝胶电泳使其分离。
	3.	Then bacteria were collected by centrifugation and split by ultrasonic method . the purified products were analyzed by tris - tricine sds - page . the immuno - activity of the recombinant proteins were analyzed by western blot 将离心收集到的菌体超声破壁，离心分离，收集上清液，进行离子交换层析和分子筛分离纯化。
	4.	Methods and results : proteomics approaches involve three critical techniques : two dimensional electrophoresis , biological mass spectrometry and bioinformatics . in 2 - de , we applied traditional ief in the first dimension and tris - tricine system in the second dimension and acquired protein profiles of serum and skin samples . in the identification of 10 proteins of serum and 16 proteins of skin , we acquired peptide mass of fingerprint ( pmf ) maps for all targets by means of matrix - assisted laser desorption - ionization time of flight mass spectrometry ( maldi - tof - ms ) and partial aminio acid sequences for 9 proteins by means of electrospray ionization ms / ms ( lisi - ms / ms ) 研究方法：采用双向电泳技术获取泥鳅创伤前后血清和皮肤的小分子蛋白图谱，利用基质辅助激光解析飞行时间检测质谱分析( matrix - assistedlaserdesorption - ionizationtimeofflightmassspectrometry , maldi - tof - ms )和电喷雾离子化串连质谱分析( electrosprayionizationms / ms , esi - ms hs )分别获得差异蛋白点的肽指纹图谱( peptidemassoffingerprint , pmf )和部分序列信息，通过互联网上的expasy服务器中和ncbi的相关软件将这些信息和swissprot数据库进行匹配鉴定蛋白质种类。
	5.	Strain bl21 , and gene expression was induced by iptg . the target proteins were directed into the periplasmic space by the staphylococcal protein a signal sequence preceding the rgd - hirudin gene . using ion exchange chromatography and gel filtration chromatography , the chimera proteins were purified , and both of them showed a single band in tricine - sds - page . the results of activity analysis suggested that these two chimera proteins not only have antithrombin activities , but gain platelet aggregation inhibitory activities as well 通过离子交换层析和凝胶过滤层分别对两种嵌合体蛋白进行纯化，纯化产物在tricine - sds - page中都显示为单一条带。活性分析结果表明两种嵌合体蛋白在保留水蛭素抗凝血酶活力的同时，还呈现抗血小板聚集活性。
	6.	Two protein peaks can be obtained by bio - gel p - 6 chromatography and both peaks have antimicrobial activity . so the bacteriocin is consisted of two proteins with different mw . only one protein with larger mw can be detected through tricine - sds - page , and its mw is about 8 , 570da 采用30硫酸铵就能完全把发酵液中的细菌素全部沉淀，通过生物胶bio - gelp - 6层析发现细菌素被分离出两条抗菌蛋白峰，这表明r21 - 4产生的细菌素是由两种不同分子量的蛋白质组成的，通过tricine - sds - page检测，只能检测到一条分子量相对较大的细菌素，分子量在8 , 570da左右。
	7.	The hwtx - i gene was chemically synthesized according to its known cdna sequence , the gene was inserted into vector ppic9k which contained aoxj promotor and the sequence of a secreting signal peptide - a - factor , the cloning ppic9k / hwtx - i was constructed and confirmed by two - step pcr and dna sequence analysis , then it was transformed into host strain gs115 , a his + muts cell line was screened and multicopy transformants were screened by various g418 concentrations , the multicopy transformant was named gh1 . gh1 was cultivated in flasks . after 6 days of induction by 0 . 5 % methanol , the supernatant was checked by 16 . 5 % tricine - sds page , which showed there was a band in the position of 3 . 5 - 6 . 1kd , then it was isolated and desalted by ultrofiltration followed by ion exchange of cm column , after reverse phase hplc of ci8 and vacuum drying , the purified rhwtx - 1 was obtained which was proved to be correct recombinant hwtx - i by tricine sds - page , maldi - tof mass spectrometry , amino acid composition analysis , the n - terminal amino acid sequence and its biological activity , the final field of the purified rhwtx - i was about 80mg / l , accounting for 23 . 6 % of it total secretory proteins 将带有hwtx -基因的ppic9k经blgii线性化后，转化酵母宿主菌gs115原生质体后经筛选阳性克隆并经表型鉴定为his ~ + mut ~ s酵母菌，进一步用遗传毒素g418筛选多拷贝的转化菌株，命名为gh1 ；将gh1甲醇酵母菌用0 . 5的甲醇诱导表达，发酵上清经90饱和度的( nh _ 4 ) _ 2so _ 4沉淀， yw - 3 ( mwc03000 )的超滤膜超滤，再经cm阳离子交换， c _ ( 18 )反相hplc纯化得到分子量为4kd左右的组分，其中4289 . 05的组分经质谱鉴定，氨基酸组成分析和序列测定为正确的表达产物，生物学活性表明其活性为天然毒素活性70 % ，表达量为80mg / l 。

Similar Words：

"tricia" Chinese translation, "tricia chen" Chinese translation, "tricia helfer" Chinese translation, "trician chen" Chinese translation, "tricin" Chinese translation, "tricing line" Chinese translation, "tricing pendants for davit" Chinese translation, "tricing pennants" Chinese translation, "tricipital" Chinese translation, "tricircuit" Chinese translation