| 1. | 2 . the functional of the purified trka extracellular domain proteins were tested using pc - 12 cells
2 、用pc12细胞株测定肠ka膜外域各结构域重组蛋白的功能。 |
| 2. | It suggests that the second ig - like domain of the trka receptors is critical for ngf binding and receptor activation
表明i夕在介导ngf与肠沁、的功能结合过程中发挥着至关重要的作用。 |
| 3. | The sds - page analysis revealed that the trka extracellular domains proteins were highly expressed and accumulated up to above 30 % of the total bacterial proteins after iptg induction
称a膜外域结构域重组蛋白表达量均占全菌蛋白的30 %以上。 |
| 4. | In present study , the recombinant subdomains of trka were expressed and purified . pc 12 cells were used to evaluate their ability to inhibit the neurite outgrowth induced by ngf
这研究，将为深入阐明， 11k受体配体识别的分子与结构机制以及研制具有药用价值的先导小分子化合物打下坚实基础。 |
| 5. | Nerve growth factor is a member of the superfamily of neutrophic factors that control the development and survival of certain neuronal populations both in the peripheral and in the central nervous system
Trka是分子量为140kda的酪氨酸蛋白激酶型受体，其分子可以分为三个部分，即膜外域，跨膜域和膜内域。 |
| 6. | In our study , the igii subdomain of trka extracellular domain was fused to pgbkt7 as a bait to screen yeast random peptide library which contains 1 107 independent random peptide clones
我们将trk受体膜外域1911结构域与gal4蛋白融合构建成pgbkt7一ign ，确认无自激活作用后，将其作为诱饵蛋白，洲于酵母双杂交筛选。 |
| 7. | However , this strategy lacks the ability of high - throughput screening . as the random screening approach requires a target molecule , it is essential to identify the subdomain of trka responsible for ngf binding and receptor activation
传统观点认为酪氨酸蛋白激酶型受体由于有较大的膜外域，小分子化合物难以使其激活，也难模拟大分子的生物的效应。 |
| 8. | Trka , the high affinity ngf receptor , contains an intracellular domain with tyrosine kinase activity and an extracellular domain with three tandem leucine - rich motifs flanked by two cysteine clusters in their amino termini and two immunoglobulin - like domains in the more membrane - proximal region
膜外域含有8个亮氨酸的重复区域，其前后分别串联着一组半胱氨酸残基，紧挨细胞膜的是两个c2 -型免疫球蛋白样区ig和ig ；膜内域为酪氨酸激酶催化活性区。 |
| 9. | Ngf binding to trka receptors results in receptor dimerization and kinase activation . recent evidence supports that not all extracellular subdomains are responsible for receptor activation . structure based drug design for neurotrophic agonists with small molecular weight relies on knowledge of the interaction of neurotrophin with their receptors
Trka在ngf作用下发生二聚化，使胞内域中酪氨酸激酶区激活，从而使trka中酪氨酸自磷酸化，并进一步激活胞内信号转导通路，从而实现神经营养信号传递。 |
