After freezing and thrawing three times , the virus is concentrated by ultracentrifugation 收集细胞培养液，并浓缩病毒。
2.
In addition , we are highly interested to study non - covalent interaction involved in the protein quaternary structure by using various biophysical methods ( including analytical ultracentrifugation and nanospray - esi - ms ) as these approaches can be applied to decipher protein - protein interaction 此外，我们也使用一些生物物理的方法，例如分析型超高速离心、电子喷?离子化质谱技术，探讨在蛋白质四级结构中的非共价性结合的交互作用，希望所得的结果能够应用在蛋白质与蛋白质的交互作用。
