Transmission electron microscopy : the testis was cut into fragments ( 1mm x 1mm x 1mm ) , fixed in 2 . 5 % glutaraldehyde made up in 0 . 1 m phosphate buffer ( ph 7 . 2 ) , postfixed in 1 . 0 % oso4 , dehydrated in a progressive ethanol and acetone solution , embedded in epon812 , sectioned with lkb ultramicrotome , and stained with urangl acetate followed by lead citrate , then observed with h - 600 microscopy and photographed 透射电镜样品以2 . 5戊二醛( ph7 . 2 ， 0 . 1m lpbs缓冲液配制)和1锇酸双固定，酒精和丙酮系列脱水， epon812包埋， lkb型超薄切片机切片，醋酸铀和柠檬酸铅双重染色， h - 600透射电镜下观察并拍照。
