| 1. | Development and microstructure of peripheral
乌骨鸡外周淋巴组织发生和结构 |
| 2. | Research on the growth curve fitness and early growth of saibei meat - type silkies
塞北肉用型乌骨鸡早期生长发育规律及生长曲线拟合研究 |
| 3. | One of them was forecasted to produce transcript factor binding site in intron 6 of silky tyrp1 . gel - shift result of this mutation proved that there is transcript factor binding site around the mutated site
经过预测，发现其中的一个snp可能使丝羽乌骨鸡的内含子6中出现了新的转录因子的结合位点。 |
| 4. | To research melanin synthesis and its accumulation mechanism , we constructed a silky bacterial artificial chromosome ( bac ) library . the library was used to study the two genes on chicken chr
为研究丝羽乌骨鸡体内黑色素合成和沉积的分子机制，本研究构建了丝羽乌骨鸡的基因组细菌人工染色体( bac )文库。 |
| 5. | ( 2 ) we searched for snps in 7 exons and intron 6 of tyrp1 by pcr and sequencing using genomic dna from 4 breeds . they are silky , white leghorn , blue shell layer and shouguang . there are 2 snps in intron 6
( 2 )在丝羽乌骨鸡、白莱航鸡、绿壳蛋鸡和寿光鸡的tyrp1的7个外显子和内含子6中寻找snp ，比较pcr产物测序结果，只在内含子6中发现两个snp 。 |
| 6. | ( 3 ) tyrp1expression was detected in chicken embryo from 0 . 5 to 16 day by rt - pcr . results showed that tyrp1 started to express in eyes of 6 day embryo , and then expressed in other tissues of 10 day embryos or later
( 3 )用rt - pcr检测丝羽乌骨鸡0 . 5到16天胚胎各组织中tyrp1的表达，结果表明tyrp1在6天的鸡胚眼中开始表达， 10天以后开始在其它组织依次表达。 |
| 7. | We designed sense and antisense primers to screen the chicken bac library of our lab , consequently we got the bac clone containing the interned sequence and used it as the probe . then by fish method we localized chicken preproghrelin at gga2q . l . 1
)设计引物，通过筛选实验室已有的丝毛乌骨鸡bac文库，继而得到含鸡preproghrelin的bac克隆，将其作为探针进行标记，用fish方法将鸡的preproghrelin定位在gga2q |
| 8. | I . results of silky bac library construction : ( 1 ) the chicken bac library consists of 138 , 240 bac clones in total ( 72 superpools , each for 20 96 - well plates ) . the stored library was organized in two grades and 3 - dimension structure , and a pcr screening system with high efficiency was built
(一)本文构建了中国特有的丝羽乌骨鸡的bac文库，结果如下： ( 1 )该文库共有138 ， 240个bac克隆(分为72个超级池，每个超级池由20块96孔板组成) 。 |
| 9. | Ii . results of study on tyrp1 and id : ( 1 ) we first sequenced the 10 , 066 bp whole genomic sequences of chicken tyrp1 . analysis of the sequences indicates that : compared with human tyrp1 , chicken tyrp1 is short of an intron and there is two microsatellites in introns
(二)本文对z染色体上与黑色素相关的基因tyrp1和id的研究结果如下： ( 1 )获得了10 ， 066bp的tyrp1的全长基因组序列，分析结果表明：丝羽乌骨鸡的tyrp1外显子与人的tyrp1外显子同源性为70 . 1 。 |
| 10. | Observation of chicken phenotype and rt - pcr results of tyrp1 expression in 8 day , 16 day embryos and 2 day hatched chicken also showed that melanin accumulation was appeared after detection of tyrp1 in silky . meanwhile , tyrpi expression was only detected in eyes of white leghorn ( except for kidney ) , the only tissue where melanin exists
Rt - pcr检测白莱航和乌骨鸡的8天、 16天胚胎和出生后2天各组织的tyrp1表达，结合对表型的观察发现，乌骨鸡各组织中黑色素的沉积随tyrp1的表达出现，而白莱航(除肾脏外)只在有黑色素沉积的眼睛中表达tyrp1 ，其他组织既无tyrp1的表达也无黑色素沉积。 |
