| 1. | Advance of application and research in - galactoxidase
半乳糖苷酶的应用研究进展 |
| 2. | Cloning and sequence of - galactosidase gene of lactobacillus burgarlus
半乳糖苷酶基因克隆和序列分析 |
| 3. | Cloning and expression of - d - galactosidase from coffee bean coffea liberica amp; amp; coffea canephora
半乳糖苷酶基因的克隆与表达 |
| 4. | The effect of - galactosidase and polygalacturonase on peach ripening and softening
半乳糖苷酶及多聚半乳糖醛酸酶对桃果实成熟软化的影响 |
| 5. | Effect of low temperature on inducing the gene expression of atgol - 3 in arabidopsis thaliana
拟南芥中低温诱导肌醇半乳糖苷合成酶基因表达作用初探 |
| 6. | The a - complementation reaction of & - galactosidase ea and ed was also used in dna cloning , protein protein interactions monitoring and expressing immunoassays studying
-半乳糖苷酶的ea 、 ed这种-互补性还被用于分子生物学、蛋白质相互作用的监控、表达免疫分析等方面的研究。 |
| 7. | P - galactosidase of e . coli which is scarce in human blood plasma and has a variety of substrates , is one of the most frequently used enzyme labels . it is used both in heterogeneous and homogeneous enzyme immunoassay
大肠杆菌的-半乳糖苷酶，因人血浆中缺乏此酶，并具有大量易得的底物，被用于均相及非均相免疫分析，是目前最常用的标记酶之一。 |
| 8. | And then a 378bp acci fragment in pbtk2 . 6 was replaced by a fragment containing the immediate early promoter of cytomegalovirus and bovine growth hormone polyadenylation signal derived from pcr3 - uni , a eukaryotic expression plasmid
在此基础上，进一步用tthlll酶切后，补平插入带有sv40启动子的大肠杆菌?半乳糖苷酶报一告基因( lacz ) ，获得通用转移载体pltk - uni 。 |
| 9. | Light microtechnique and sa - galactosidase method was used to study the effects of super - high - concentration of glucose on the senescence of human diploid fibroblast 2bs cells , ros and the membrane potential of mitochondria during this process were measured . our results showed that 200 mmol l of glucose inhibited the growth of 2bs cells , led to the changes of reactive oxygen species and decrease of mitochondrial membrane potential , and caused senescence of 2bs cells rapidly . it supports the hypothesis of oxidative damage of senescence . moreover it is a better system for the study of the effects of ros during the process of replicative senescence
利用光学显微镜观察和酸性-半乳糖苷酶染色技术研究了高浓度葡萄糖对人二倍体成纤维细胞2bs细胞衰老进程的影响，并用流式细胞仪检测了此过程中活性氧和线粒体膜电位差的变化。结果表明： 200 mmol l的葡萄糖对2bs细胞有生长抑制作用，能引起活性氧含量的变化，导致线粒体膜电位差显著下降，并诱导了细胞的衰老。这为氧化损伤假说提供了新的证据，并为研究活性氧和复制衰老之间的关系提供了较好的体系。 |
| 10. | The gpa1 gene was obtained via pcr amplification and was cloned in the two - hybrid dna binding domain vector pgbkt7 the combinant plasmid was designated as pgbkt7 - ga . - galactosidase assay indicated that got did not have the property of self - activation . after pgbkt7 - ga was transformed to yeast pj69 - 4a , we transformed arabidopsis vegetative tissue two - hybrid cdna library plasmids to yeast pj69 - 4a containing pgbkt7 - ga
通过pcr扩增得到gpa1基因并将其克隆到双杂交dna结合域载体pgbkt7中，得到的重组质粒命名为pgbkt7 - g ， ?半乳糖苷酶活性鉴定表明g不具有自激活特性，将其转化到酵母菌pj69 - 4a中，再以此为受体菌转化拟南芥绿色营养组织cdna文库质粒。 |
