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Home > english-chinese > "体转" in Chinese

Chinese translation for "体转"

side stretch
Example Sentences:
1.Gfp expression was monitored using a fluorescence microscope . the result showed that the fusion gene was expressed at a low level
利用脂质体转染的方法在美国棉铃虫细胞hz中表达了gfp - actin融合基因。
2.We generated a recombinant eukaryotic gene expressing vector harboring a full - length hgh gene , 2 . 4 kb genomic dna with four introns and the signal peptide sequence cloned to the eukaryotic gene expressing vector pcdna3 . 0
我们直接将含有4个内含子和自身信号肽的2 . 4kb全长人生长激素基因直接克隆至真核表达载体pcdna3 . 0 ,然后利用脂质体转染家蚕bmn细胞,瞬时表达hgh 。
3.Recombinant fpvs were selected and purified by blue plaques expressing 3 - galactosidase . . the expression of foreign genes in rfpvs were confirmed by ifa . trails for evaluating protective efficacy of rfpvs against very virulent mdv or aiv challenge
纯化后脂质体转染,蓝斑筛选纯化得到稳定的重组病毒rfpv - ps - ha - pe / l - f ,间接免疫荧光法证实, ha基因和f基因同时得到了表达。
4.In this study , the recombinant fowl - poxvirus was transfected into expressing the vp3 gene of isolated gpv h1 strain into the cef cells with fpv - 017 by liposome , which have the lacz reporter gene , earlier / latter promoters lp2ep2 of fpv , promoters p7 . 5 and p7 . 1 of vaccinia virus , replication unnecessary region of fpv - 017 . following 6 cycles screenings , clonings , purification of blue plaques , detection of pcr and dot - elisa , which verified the genetic stable vp3 - fowlpox virus recombinant constructed successfully . this study provided the theoretical and practical foundation for development of gpv recombinant fowl - poxvirus genetic engineering vaccine , as well as provided substance preparatory for prevention the high mortality gpv
本研究采用脂质体转染方法,将含有完整gpvh1分离株vp3基因、报告基因lacz 、禽痘病毒早晚期启动子lp2ep2 、痘苗病毒启动子p7 . 5 、 p11和fpv - 017复制非必须区的转移载体质粒psy681vp3lacz与fpv - 017共转染鸡胚成纤维细胞,经6轮蚀斑克隆、筛选、表达, pcr鉴定和dot - elisa检测,证明该重组病毒已构建成功,并获得了遗传性状稳定的鹅细小病毒vp3基因的重组禽痘病毒。
5.The plasmids pci - mbl54 containing full length of mutations mbl cdna were propagated hi escherichia coli xl - 1 blue , then the extracted and purified pci - mbl54 were used to transfect dhfr ( - ) chinese - hamster ovary ( cho ) cells . after screeened with g418 and cloned , 4 g418 - resistent clones were randomly selected for detection of mrna expression by rt - pcr and molecular beacons . it was found that all of the 4 positive cell clones expresse mbl analogue as detected in transcription level
抽提、鉴定、纯化重组质粒后,脂质体转染法将重组质粒导入中国仓鼠卵巢细胞( cho - dhfr ~ - )中, g418选择转染子并克隆化培养,经rt - pcr和分子灯塔探针杂交鉴定其mrna转录,获得4株稳定表达54位密码突变型mbl的cho细胞。
6.After the recombinant plasmid pcdna3 . 1 / ts87 was identified by digestion of hindlll and bamh i , it transformed into cos7 by lipofectamine . expression product was identified by immunohistochemical method , sds - page and western - blot . the immunocytochemistry result has shown that specific brown - staining grains were found in the cytoplasm of cells transformed by recombinant plasmid versus not seen in cells transformed by pcdna3 . 1 or normal cells ; the sds - page result has revealed that a band about 3 8kb was found in cell lysis transformed by recombinant plasmid versus not in cells transformed by pcdnas . l or normal cells ; the western - blot result has showed that only the band about 38kd was recognized by sera from rabbit infected by t . s artificially and sera from rabbit immunized with soluble antigen of t . s and with protein expressed by ts87 gene and by a monoclonal antibody of t . s
通过细胞的免疫组化,细胞裂解物的sds - page电泳, westem - blot分析检测目的基因的表达情况。免疫组化结果显示:重组质粒转染的细胞质中有棕褐色颗粒,而空载体转染细胞及正常细胞无此现象;细胞裂解物sds - page电泳结果显示:只有重组质粒转染的细胞在约38kd处有明显的蛋白带,这与理论计算的ts87基因表达蛋白的分子量为38kd基本一致; western - blot分析结果显示:约38kd的蛋白带能够分别被旋毛虫感染兔血清,成虫虫体可溶性抗原免疫兔血清, ts87基因原核表达蛋白免疫兔血清( ts87血清)以及一株具保护性的旋毛虫单抗特异识别。
7.2 . construction of chimeric mtb8 . 4 / hil - 12 eukaryotic expression plasmid ( 1 ) construction of pci - neo - mtb8 . 4 - linker ( pml ) and pci - neo - ms - linker ( pmsl ) mtb8 . 4 - linker and ms - linker gene ( without stop codon ) were pcr amplified by using two oligonucleotides designed to generate nhe i and mlu i restriction sites at the 5 " and 3 " ends of the amplified fragments , respectively
3 .重组质粒在真核细胞中的表达: pm 、 pms 、 pmi和pmsl重组质粒用lipofectaminatmzo0o脂质体转染试剂转染cos一7细胞,进行瞬时表达, 48小时后,用rl ’ - pcr检测目的基因在mrna水平的表达;用westemblotting检测hil一12在蛋白质水平的表达。
8.However , only c - myc protein was slightly increased in cells transfected with vector alone and other proteins expression level were the same as conditions in the absence of tgf - 1 . in addition , hepg2 cells transfected with pcdnas . 1 / ha - axudl plasmid were arrested in gl phase compared with the cells transfected with vector alone
另外,流式细胞仪检测表明, ha一axudi蛋白的过表达细胞组较空载体转染细胞组, g0 / g1 ( % )上升,而s ( % )下降,说明细胞阻滞于g1期。
9.In order to study the function of cycling2 in vitro culturing cell line , we used pires - g2 eukaryotic expression vector transfecting human gastric cell line sgc - 7901 and human embryo kidney hek - 293 cells by lipofectamine plus reagent , and studied the function of cycling2 expression on the cell proliferation in vitro , further investigated the regulation mechanism of cycling2 . at the same time , we made a study on the expression level change of cycling2 in normal gastric tissue and different type and different stage of gastric carcinoma tissue . material and method 1 material : piresneo vector was purchased from clonetech , plasmid extraction and purification kit was purchased from qiagen company ; rpmi 1640 , , dmem fetal calf serum were obtained from gibco / brl ; lipofectamine plus and g418 were purchased from life technologies ; ultrasensitive ? s - p kit , mouse monoclonal antibody p21wafl ( in use ) , dab staining kit were purchased from maixin company
实验材料与方法1 .实验试剂高糖dmem 、 rpmll640和胎牛血清购自美国g山eo / brl公司; dmewf12 ( 1 : 1 )混合培养液购自美国hyclone公司;胰蛋白酶购自美国si目叮a公司; hepes由美国amersco公司分装;脂质体转染试剂( upofectalnineplusreageni )和以18为美国玩vitrogen公司产品; piresneo载体购自美国cloneteeh公司;质粒提取及纯化试剂盒购自德国qiagen公司; ultresensitive翎s一p免疫组织化学试剂盒;鼠单克隆抗体户3 ( do一7 )蛋白(即用型) ;鼠单克隆抗体p21waf , (即用型) ; dab染色试剂盒均购自福建迈新公司;鼠单克隆抗体pziwa曰(浓缩型) ;辣根过氧化酶标记羊抗鼠二抗购自北京中山公司; ecl试剂盒购自美国santacruze公司; dcproteinassay试剂盒购自bi 。
Similar Words:
"体轴系纵轴" Chinese translation, "体轴下的" Chinese translation, "体轴形成" Chinese translation, "体轴原点" Chinese translation, "体珠海猪鱼" Chinese translation, "体壮如牛" Chinese translation, "体锥面" Chinese translation, "体姿" Chinese translation, "体姿稳定" Chinese translation, "体姿协调" Chinese translation