| 1. | Es cells are the cells derived from the inner cells mass ( icm ) . they are capable of differentiating in vitro into multiple cells types under the stimulation of right signals
Es细胞来源于着床前囊胚期内细胞团( innercellmass , icm ) ，具有发育全能性。 |
| 2. | Inner cell mass ( icm ) of the blastocyst stage embryo and primordial germ cells ( pgcs ) of fetus are the mostly used materials for derivation of pluripotent stem cells
早期囊胚的内细胞团( innercellmass ， icm )和原始生殖细胞( primordialgermcells ， pgcs )是获得多能干细胞( pluripotentstemcell )的两种主要的材料来源。 |
| 3. | Purpose embryonic stem cells ( escs ) which are from the inner cell mass ( icm ) of preimplantation blastula are pluripotent . neurons are considered as terminally differentiated cells that can not divide nor proliferate after injury
目的胚胎干细胞( embryonicstemcells ， escs )来源于着床前胚胎的囊胚期内细胞团( innercellmass ， icm ) ，具有发育全能性。 |
| 4. | Although the cell parameters ( icm and tcn ) of blastocysts of both groups differed significantly ( p < 0 . 05 ) , the mml 6 + e2 + p group was the closest to the in vivo group , and those of the mm16 group were the lowest
同时添加e2和p的mm16培养液中囊胚的细胞总数、内细胞团数、内细胞团占总细胞的百分率和内细胞团与滋养层细胞数的比率低于体内正常发育囊胚，但显著高于其它体外培养组( p 0 . 05 ) 。 |
| 5. | After 4 days of growth , the intact inner cell mass ( icm ) were separated with 0 . 05 % trypsin - edta and replated on feeder layer in dmem containing 15 % serum , 0 . 1mmol / l nonessential amino acids , 0 . 1mol / l 2 - mercaptoethanol , 2mmol / l glutamine , 100 units / ml of streptomycin and 100 units / ml of penicillin . after 4 - 6 days 5 es cell colonies were selected and expanded in which alkaline alkaline phosphatase was detected
( 2 )小鼠囊胚或内细胞的培养和es细胞的分离培养了156个不同品系的小鼠囊胚，经过3 ? 4天培养后，将增殖出来内细胞团用机械法结合胰酶- edta处理，离散后培养于mef饲养层上， 4 6天后有5例出现了es细胞样集落。 |
| 6. | Embryonic stem ( es ) cells were derived from the inner cell mass ( icm ) of preimplantation embryos , and embryonic germ ( eg ) cells from primordial germ cells ( pgc ) . both es and eg cells were cultured to determine the factors affecting on isolation , cloning and passage of pluripotent stem cells from icr mouse embryos
试验以icr小鼠胚胎内细胞团( icm )和原始生殖细胞( pgc )为材料，采用不同培养体系分离克隆胚胎多能干细胞，探讨icr品系小鼠胚胎多能干细胞分离克隆的基本方法，以及一些因素对其分离、培养、克隆、传代等效果的影响。 |
| 7. | The blastocyst was obtain from the mouse after 3 . 5 days of pregnancy , and was cultivated on the mouse embryonic flbroblast feeder layer . the blastocyst usually attach to the feeder layer after 48 hours , then the inner cell mass began to grow and form a big cell mass of embryonic stem cells . these cells can form cell clones with the conformation of embryonic stem cell
囊胚一般在48小时后贴壁并脱去透明带，由囊胚中的内细胞团增殖形成一个胚胎干细胞球，待其生长至一定程度后对其进行酶解传代，在传代后的细胞中重新生成es集落形态的细胞集落，在集落生长至一定程度后再进行传代，此后大约每5 ? 7天传代一次。 |
| 8. | The percentage of blastocysts in ml 6 medium with both taurine and edta ( 73 . 6 + 2 . 4 % ) was significantly higher than that in m16 with edta and taurine alone ( 60 + 1 . 0 % and 61 . 9 + 2 . 0 % , respectively ) . the change pattern in the cell parameters ( inner cell mass , icm ; total cell numb , tcn ) of the three groups was the same as the blastocyst formation rate
且m16 + tau + edta培养液中的囊胚总细胞数、内细胞团数、内细胞团与滋养层细胞数的比率均显著高于m16 + tau和m16 + edta培养液的( p 0 . 05 ) ；内细胞团占总细胞数的百分率也相应增加。 |
| 9. | Embryonic stem ( es ) cells are pluripotent cells derived from the inner cell mass ( icm ) of early blastrocyts , which possess the ability of unlimitted proliferation and maintain normal dipoid keptypes under differentiation - inhibited culture in vitro , they will differentiate into multiple lineage cell types or occur commentent and differentiation without differentiation - inhibited factors or hi presence of certain induced - factors .
胚胎干细胞( es细胞) ，是存在于早期胚胎内细胞团( icm )的一种多潜能细胞。在体外抑制分化培养时， es细胞可无限增殖并保持正常的二倍体核型，一旦撤除分化抑制因素，或添加适当诱导剂， es细胞可发生谱系分化或定向分化。 |
| 10. | Since the first embryonic stem cell derivation from mouse blastocyst by british scientists in 1981 , groups of scientists were devoted into the research field of stem cells . in 1998 , two scientific groups of america obtained human embryonic stem cells and human embryonic germ cells respectively from human blastocysts and gonadal ridge of early embryos
1998年，美国的两个科学家小组分别从人体外受精发育而成的囊胚内细胞团和5 9周龄流产的胚胎原始生殖嵴中，成功获得了人类胚胎干细胞系和人类生殖嵴干细胞系。 |
