| 1. | Advance of application and research in - galactoxidase
半乳糖苷酶的应用研究进展 |
| 2. | Cloning and sequence of - galactosidase gene of lactobacillus burgarlus
半乳糖苷酶基因克隆和序列分析 |
| 3. | Cloning and expression of - d - galactosidase from coffee bean coffea liberica amp; amp; coffea canephora
半乳糖苷酶基因的克隆与表达 |
| 4. | The effect of - galactosidase and polygalacturonase on peach ripening and softening
半乳糖苷酶及多聚半乳糖醛酸酶对桃果实成熟软化的影响 |
| 5. | The a - complementation reaction of & - galactosidase ea and ed was also used in dna cloning , protein protein interactions monitoring and expressing immunoassays studying
-半乳糖苷酶的ea 、 ed这种-互补性还被用于分子生物学、蛋白质相互作用的监控、表达免疫分析等方面的研究。 |
| 6. | P - galactosidase of e . coli which is scarce in human blood plasma and has a variety of substrates , is one of the most frequently used enzyme labels . it is used both in heterogeneous and homogeneous enzyme immunoassay
大肠杆菌的-半乳糖苷酶，因人血浆中缺乏此酶，并具有大量易得的底物，被用于均相及非均相免疫分析，是目前最常用的标记酶之一。 |
| 7. | And then a 378bp acci fragment in pbtk2 . 6 was replaced by a fragment containing the immediate early promoter of cytomegalovirus and bovine growth hormone polyadenylation signal derived from pcr3 - uni , a eukaryotic expression plasmid
在此基础上，进一步用tthlll酶切后，补平插入带有sv40启动子的大肠杆菌?半乳糖苷酶报一告基因( lacz ) ，获得通用转移载体pltk - uni 。 |
| 8. | Light microtechnique and sa - galactosidase method was used to study the effects of super - high - concentration of glucose on the senescence of human diploid fibroblast 2bs cells , ros and the membrane potential of mitochondria during this process were measured . our results showed that 200 mmol l of glucose inhibited the growth of 2bs cells , led to the changes of reactive oxygen species and decrease of mitochondrial membrane potential , and caused senescence of 2bs cells rapidly . it supports the hypothesis of oxidative damage of senescence . moreover it is a better system for the study of the effects of ros during the process of replicative senescence
利用光学显微镜观察和酸性-半乳糖苷酶染色技术研究了高浓度葡萄糖对人二倍体成纤维细胞2bs细胞衰老进程的影响，并用流式细胞仪检测了此过程中活性氧和线粒体膜电位差的变化。结果表明： 200 mmol l的葡萄糖对2bs细胞有生长抑制作用，能引起活性氧含量的变化，导致线粒体膜电位差显著下降，并诱导了细胞的衰老。这为氧化损伤假说提供了新的证据，并为研究活性氧和复制衰老之间的关系提供了较好的体系。 |
| 9. | The gpa1 gene was obtained via pcr amplification and was cloned in the two - hybrid dna binding domain vector pgbkt7 the combinant plasmid was designated as pgbkt7 - ga . - galactosidase assay indicated that got did not have the property of self - activation . after pgbkt7 - ga was transformed to yeast pj69 - 4a , we transformed arabidopsis vegetative tissue two - hybrid cdna library plasmids to yeast pj69 - 4a containing pgbkt7 - ga
通过pcr扩增得到gpa1基因并将其克隆到双杂交dna结合域载体pgbkt7中，得到的重组质粒命名为pgbkt7 - g ， ?半乳糖苷酶活性鉴定表明g不具有自激活特性，将其转化到酵母菌pj69 - 4a中，再以此为受体菌转化拟南芥绿色营养组织cdna文库质粒。 |
| 10. | The gardenia bule withdraws by the rubiaceae plant gardenia fruit practical lukewarm water obtained assumes the color glucoside and the protein resolvent mixture , adds beta - the galactose glucoside enzyme after the fermentation , the deactivation , filters , evaporates , fine , is dry but becomes , assumes the blue color powder
栀子蓝色素由茜草科植物栀子果实用温水提取所得的呈色配糖体和蛋白质分解物的混合物，加-半乳糖苷酶经发酵、灭活、过滤、蒸发、精致、干燥而成，呈蓝色粉末。 |
