| 1. | Isolated microspore culture is one of the methods to obtain haploid
摘要游离小孢子培养技术是获得大白菜单倍体植株的有效途径之一。 |
| 2. | Doubling efficiencies of the application dimethylsurfoxide in colchicines to treat haploids in tobacco nicotiana tabacum
对烟草单倍体植株的染色体加倍效应 |
| 3. | Gynogenic haploids through unfertilized ovules and ovaries have been reported from 21 species in 9 families since 1976
摘要介绍被子植物未受精胚珠与子房离体培养诱导单倍体植株的研究进展。 |
| 4. | With haploid as transgenic material can decrease mosaic and can avoid properties separation in transforming
采用单倍体植株作为遗传转化的材料会避免转基因过程中的嵌合体问题和子代分离问题。 |
| 5. | By means of pcr amplification to total dna extracted from four transgenic plants , it indicated that bet - a gene had been integrated into the haploid of the hybrid genome
最终得到四个转化株系，经pcr检测，全部产生特异性扩增谱带，证明bet - a基因已整合进小黑杨单倍体植株的基因组中。 |
| 6. | Embryological studies reveal that megaspore and such cells as egg , synergid and antipodals in mature embryo sac initiate the division of forming haploid plants through embryogenesis or callus formation
胚胎学观察揭示大孢子与胚囊内的卵细胞、助细胞和反足细胞均有可能在培养中启动分裂，通过胚状体或愈伤组织形成单倍体植株。 |
| 7. | Anther culture ( pollen culture ) the generation of haploid plants from immature pollen grains or intact excised anthers . the resultant plants are generally smaller than their diploid counterparts
花药培养：从未成熟的花粉粒或者切下的完整的花药中再生出单倍体植株的过程。这样培养出来的单倍体植株通常比正常培养出的二倍体植株小。 |
| 8. | This review includes the following subjects : induction frequency of haploids depend on genotypes , developmental stage of explants , temperature pretreatment of the explants , medium composition and the conditions of cultivation
迄今己有9个科21种植物用这一方法诱导出单倍体植株，植物的基因型、外植体的发育程度、接种前的预处理、培养基和培养条件等均能影响诱导率的高低。 |
| 9. | Choline dehydrogenase ( cdh ) isolated from escherichia coli , can help the expression of glycine betaine efficiently . to get some salt - tolerant plants , the cdh gene , mediated by agrobacterium , was transformed to the haploid of populus simoniixp . nigra
本实验以农杆菌( agrobacteriumtumefaciens )为介导进行遗传转化，将编码cdh的耐盐基因( bet - a )导入小黑杨( populussimonii p . nigra )单倍体植株( haploid )的基因组内，以期获得耐盐植株。 |
| 10. | Based on the advances that many experts had studied in flax anther culture field for thirty years in country and abroad , some aspects were summarized and discussed as followed : ( 1 ) selection and manufacture of anther medium of flax ; ( 2 ) effect factors of anther culture ; ( 3 ) haploid plant appraisal ; ( 4 ) haploid variety breeding and application in anther culture breeding
摘要在总结三十多年国内外学者在亚麻花药培养研究领域所取得的研究成果的基础上，从以下几个方面进行了综述与讨论： ( 1 )亚麻花药培养基的筛选及研制； ( 2 )影响亚麻花药培养因素的研究； ( 3 )单倍体植株的鉴定； ( 4 )单倍体品系（种）的选育及花药培养在育种中的应用。 |
