| 1. | Detection of arabis mosaic virus by one - step rt - pcr
检测南芥菜花叶病毒方法的建立 |
| 2. | The results showed that the fragment was 890bp , nearly consistent to the sequence of p - rd29a isolated from arabidopsis thaliana ( d13044 )
结果表明，该目的片段为890bp ，与发表的拟南芥菜p - rd29a ( d13044 )相应序列一致。 |
| 3. | Furthermore , rice is only the second plant to have its genome sequenced extensively , and thus comparisons to the previously sequenced arabidopsis thaliana genome will be of great interest to a wide range of plant scientists
除此之外，水稻基因组是迄今为止第二个精确测序的植物基因组，因此对于植物方面的科学家而言，将它和之前完成的拟南芥菜基因组的比较是一件非常有意思的事。 |
| 4. | A . thalianas were respectively introduced by ion beam with a collection of donor dna having a gradient genetic relation with a . thaliana . in vegetative phase of tl , the ratio of phenotypic variation showed no distinct difference among these transformed populations
用离子束介导与拟南芥菜亲缘关系从近到远一系列的外源供体的全dna转化拟南芥菜，在转化当代营养生长期，各转化群体的表型变异情况没有明显的差别。 |
| 5. | Two variant lines , named by tc243 - 1 and tc243 - 2 , had been obtained and their variant traits had been steadily inherited to t5 . the query to nasc suggested that tc243 - 1 and tc243 - 2 were new mutants . the molecular analysis of transferred plants and their offspring was carried out from the following four aspects
在离子束介导红甜菜转化后代中筛选到两个稳定变异株系tc243 - 1和tc243 - 2 ，经与拟南芥菜突变体库比较，确认为新的变异类型。 |
| 6. | The constructed clone vector was defined as pcbf3 and sequenced by sangon bio - engineering co . ltd . the results showed that the fragment was 760bp , encoding 216 amino acid and consistent to the sequence and amino acid of cbf3 isolated from arabidopsis thaliana ( af076155 )
获得的重组质粒命名为pcbf3 ，委托上海生工生物工程有限公司测序。结果表明，所克隆的目的片段为760bp ，编码216个氨基酸，与发表的拟南芥菜cbf3基因( af076155 )相应序列及编码的氨基酸一致。 |
| 7. | The volatile oil of a . thaliana transferred with mint total dna was analyzed by means of gc - ms . two transgenic plants were measured to contain menthol component in 250 plants of tl generation and one plant in then * 267 progenies , which provided explicit evidence for ion beam - mediated polygenic character transmission between distant species . two conclusions were drawn : one was that multiple genes controlling the same trait could be transformed into the receipt and efficiently expressed , the other was that the transferred new character was hereditary , i . e . , the genes controlling the new character could inherit
用gc - ms分析了离子束介导薄荷全dna转化的拟南芥菜个体的挥发油成分，在250个转化当代植株中有两株被测出含有薄荷醇，在这两株的267个t2代植株中仍有1株被测出含有薄荷醇，该实验为离子束介导外源全dna转化实现物种间多基因复杂性状的转移提供了明确的证据。 |
| 8. | Mint total dna was transferred into a . thaliana mediated by ion beam with the influence of 0 . 5 10 1 . 5 1017 and 2 . 5 1017 ions / cm2 , respectively , which were on the bridge of saddle . among three transferred populations , their budding , seedling , growth and phenotypic variation took on obvious difference . combining these facts , the influence of 1 . 5 1017ions / cm2 was decided as transformation influence of the following work
我们从另外一个角度出发，在离子注入剂量-拟南芥菜存活曲线的基础上，选择不同的离子剂量介导薄荷全dna转化拟南芥菜，根据各个转化群体在遗传和生理上的不同变化，选择1 . 5 10 ~ ( 17 ) ions cm ~ 2作为我们以后转化工作的转化剂量。 |
| 9. | Sequence alignment and southern blot showed that this band was not the sequence of a . thaliana genome , but from exogenous dna donor cabbage genome . it could be served as direct molecular evidence that the exogenous dna could be transferred into receipt mediated by ion beam . further analysis showed that this exogenous dna fragment had changed in structure during the transformation
，该条带的dna序列和拟南芥菜基因组序列库的比对结果表明，该条带不是拟南芥菜基因组本身的dna序列， southem杂交结果表明该条带来自转化供体甘蓝基因组，找到了外源全dna转化受体的分子证据，进一步分析表明该外源片段在转化过程中发生了结构变化和修饰。 |
| 10. | Genes encoding the plant glutamate receptors were isolated from arabidopsis thaliana , a weed that has been adopted as a genetic model for plant researches . their study demonstrated that glutamate receptors in plants are also involved in a complex signalling pathway . when plants were grown in the presence of a compound that blocks human glutamate receptors , the plants response to light was partially hampered as illustrated by longer hypocotyl and reduced level of chlorophyll
他们在拟南芥菜( arabidposisthaliana ,一种植物遗传学研究常用的野草)中分离出?氨酸感受器的基因编码，并发现这些感受器直接影响植物内的讯息传递当植物种植在含有?氨酸感受器抑制剂的培养基内，植物的感光反应即受影响，其下胚轴会增长而叶绿素的合成则减少，由此证明植物内光的讯息传递受?氨酸感受器调控。 |
