| 1. | Establishment of high frequency regeneration system for tissue culture of alfalfa
家畜类腔前卵泡的体外分离方法和培养体系研究进展 |
| 2. | The basement membrane of follicles isolated by m1 remained integrity as identified by electron microscope
电镜分析结果表明， m1法不损伤卵泡的基膜。 |
| 3. | Cpges mrna was strongly expressed in granulosa cells of primary and secondary follicles
Cpgesmrna在初级卵泡和次级卵泡的颗粒细胞上均有较强的的表达。 |
| 4. | The number of granulosa cells in primordial and primary follicles were 10 . 8 2 . 3 and 18 . 1 3 . 1 respectively
( 2 )对水牛腔前卵泡的分离方法进行了系统摸索。 |
| 5. | Mpges mrna was expressed in granulosa cells of primary and secondary follicles during ovulation induced by gonadotropin
促性腺激素诱导的小鼠排卵模型中， mpgesmrna在初级卵泡和次级卵泡的颗粒细胞上均有表达。 |
| 6. | As the follicular diameter increases , the survival rate and growing extent of follicles increases , the morphological abnormity of follicles decreases
发现随着卵泡的直径增加，体外存活率升高，增长的幅度上升，异常率下降。 |
| 7. | The present studies were conducted to investigate the distributing and the histological character of buffalo preantral follicles in ovary , and explore a method for isolation and culture of buffalo preantral follicles
本研究对水牛卵泡在卵巢中的分布规律及组织学特征、水牛腔前卵泡的分离与培养方法进行了系统研究。 |
| 8. | Three dimensions culture system is proved to be suitable for cultunng the follicles in diameter of 60 ~ 100 m , while the two dimension culture system is proved to be suitable for culturing the follicles in diameter of 100 - 140 m
结果发现，三维培养法适宜培养60 100 m的卵泡，而二维法有利于100 140 m卵泡的体外发育。 |
| 9. | There was detectable mpges immunostaining in the granulosa cells of the different follicles . the strongest mpges immunostaining was seen in the primordial follicles , while a strong level of the immunostaining was also observed in the primary follicles , the secondary follicles , and mature follicles
在原始卵泡、初级卵泡、次级卵泡及成熟卵泡的颗粒细胞中都有mpges免疫染色，其中原始卵泡中最强，初级卵泡、次级卵泡和成熟卵泡中较强。 |
| 10. | The result are summarized as follows : 1 . the surface diameter and the dissected diameter ( measured after the follicles were dissected from the ovary ) of follicles larger than 1 . 5 - 2 . 5mm in diameter were closely correlated ( r = 0 . 72 ) , and therefore we can classify follicles in accordance with the surface diameter
结果表明： 1 、山羊卵巢表面直径1 . 5 - 2 . 5mm以上卵泡的表面直径和实测直径具有显著相关性，可以利用其表面直径对卵泡进行分类。 |
