Chinese translation for "原核载体"
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- prokaryotic vector
Related Translations:
- Example Sentences:
| 1. | A 1 . 7kb fragment encoding ge of prv fa strain was obtained by pcr from plasmid ppge templated using a pair of the designed primers containing ecori and bamhi ' sites . the ge gene fragment cutted with ecori and bamhi was inserted into the expression plasmid pbv220 including these two endonuclease sites for constructing the recombinant plasmid pbvge . strain dh5a of e . coli contain pbvge was induced at 42 for 4 - 6hr after incubation with vigorous shaking at 30 for 3hr or so 以质粒ppgedna为模板, pcr扩增出1 . 7kb的ge基因完整片段,将扩增产物以ecori和bamhi双酶切后,插入原核表达载体pbv220的p _ rp _ l启动子下游的ecori和bamhi位点间,得到重组表达质粒pbvge ,转化了pbvge的大肠杆菌dh5a经温敏诱导表达后,用sds - page和western - blot ,以及琼脂双扩散来检测,结果表明prvfa株ge基因在原核载体上得到高效表达,表达产物约占总蛋白的17 。 |
- Similar Words:
- "原核系统" Chinese translation, "原核细胞" Chinese translation, "原核细胞界,原核生物界" Chinese translation, "原核细胞型微生物" Chinese translation, "原核原生生物" Chinese translation, "原核质的" Chinese translation, "原核质体" Chinese translation, "原合同" Chinese translation, "原合同号" Chinese translation, "原合同年龄转换方式" Chinese translation
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