| 1. | Inhibitory effects of beta - elemene cooperating with anti - cd3 anti - glioma bispecific antibodies on growth of neuroglioma
抗胶质瘤双特异性抗体抑制神经胶质瘤生长 |
| 2. | Fortunately , recently widely used bispecific antibody technique provided us a good model for target rca blocking for it ' s dual antigen binding activity and for the availablity of high
为了进一步探讨用基因工程的方法制备抗病毒双特异性抗体的可能性，我们又在现有的条件下构建、表达了 |
| 3. | Objection : this study is to prepare a kind of bispecific antibody of anti - hiv p24 antigen and anti - human rbc type a antigen , and to build a new way to detect hiv p24 antigen with this bispecific antibody
目的：制备抗hivp24和抗人a型红细胞双特异性抗体，建立检测hivp24抗原的新方法。 |
| 4. | The results of the first part showed that both cd55 and cd59 neutralizing antibody can enhance the lysis or neutralization activity of complement . however , since rca are distributed widely , techniques that can mediate target rca blocking on virions are necessary
最后，病毒中和功能实验结果显示，我们制备的双特异性抗体具有良好的诱导补体活化，中和eev病毒感染的作用，与亲本抗体相比有增强的eev病毒中和活性。 |
| 5. | Therefore the hybrid - hybridoma can secrete not only bispecific antibody but also anti - human rbc type a monospecific antibody and probably anti - p24 monospecific antibody . the mixed antibodies purified by ammonium sulfate is further purified by p24 affinity chromatography . the unsp
这样，分离出双特异性抗体，并证实杂交杂交瘤除分泌抗p24和人红细胞的双特异性抗体外，还分泌抗p24单特异性抗体和抗人a型红细胞单特异性抗体。 |
| 6. | This bispecific antibody companied with another anti - p24 monospecific monoclonal antibody can build a new way of indirect rbc agglutination to detect p24 antigen , and this new way is preliminarily applied to detect serial diluted p24 to determine the sensitivity
用双特异性抗体致敏人a型红细胞做指示剂，以抗p24单特异件单克隆抗体9a7做交联剂，建立间接血凝检测p24抗原的新方法，并初步应用于检测系列稀释的p24抗原以确定测定的敏感性。 |
| 7. | 2 - e4 and s2 is induced respectively by 8 - ag and 5 - brdu with different drug concentration to make them deficient in hypoxanthine - guanine phosphoribosyl transferase ( hgprt ) and in thymidine kinase ( tk ) respectively and renamed 2 - e4 - a and 82 - 6 . their antibodies " isotypes are tested by goad anti - mouse isotype regent
取驯化好并处于对数生长期的2 - e _ 4 - a和s _ 2 - b细胞，再常规融合和筛选，三次克隆化后得稳定分泌双特异性抗体的杂交-杂交瘤细胞株6株。 |
| 8. | The bispecific antibody tilers of ascites fluid and culture supernatant of 3 - hn are 1 : 16000 and 1 : 64 . at the same time the anti - p24 antibody and the anti - human rbc type a antibody in the ascites fluid of hybrid - hybridoma 3 - hn are tested , and their titer is 1 : 800 and 1 : 16000 respectively
为89士9条j十； ；的腹水型和上清型双特异性抗体的效价分别为1 ： 16000和1 ： 64 ，同时测定腹水型抗体抗p24抗体活性及抗人a型红细胞凝集抗体活性，效价分别为1 ： 800和1 ： 16000 。 |
| 9. | The ascites fluid antibody purified by 33 % ammonium sulfate is further purified by chromatography . the p24 affinity chromato - graphic column done to purify the bispecific antibody is in the brcn activated sepharose 4b . after washing the unspecific proteins , change the washing buffer and the anti - p24 / anti - human rbc type a bispecific antibody and the anti - p24 monospecific antibody are gained respectively
方法是先用硫酸绥盐析，再过亲和层析柱，即33饱和硫酸按沉淀后，透祈过夜，上p24抗原做配体的滨化氰活化的sepharose4b柱，流洗掉杂蛋白和抗人红细胞单特异性抗体后，再洗脱并收集双特异性抗体。 |
| 10. | 2 - e4 - a and 82 - 6 are hybridized during their log growing time , and the hybrid - hybridomas are cloned for 3 times and produce 6 hybrid - hybridoma cells . the chromatosome of hybrid - hybridoma 3 - hu and hybridoma 2 - e4 - a and s2 - b are counted , and the antibody of ascites fluid or culture supernatant of 3 - hn is prepared . the positive clones are detected by three methods at the same time : rbc agglutination for monospecific anti - human rbc type a antibody , indirect elisa for anti - p24 antibody , and rbc solid - phase adherence for bispecific antibody
选其中一株3 - h _ ( 11 )做杂交-杂交瘤细胞染色体计数，同时计数两母株2 - e _ 4 - a和s _ 2 - b的染色体数：制备腹水型和上清型抗体，用三种方法同时检测其中的双特异性抗体、单特异性抗人红细胞抗体和抗p24抗体，即红细胞固相吸附法测双特异中文摘要性抗体，红细胞凝集试验测单特异性抗人a型红细胞抗体，间接elisa法测抗p24抗体；用腹水型抗体做耐热性及耐冻融实验。 |
