| 1. | Isolation and enzyme screening of halophiles from ayakekum lake
阿牙克库木湖嗜盐菌的分离及功能酶的筛选 |
| 2. | Study on halibacteria growth disciplinarian under desalinated seawater condition
嗜盐菌在浓海水条件下生长规律的研究 |
| 3. | Isolation and degradation characteristics of polychlorinated biphenyls - degrading halophilic bacteria
降解多氯联苯嗜盐菌的分离和降解特性 |
| 4. | Purple clones were picked out from the plate , which show br was expressed . pcr analysis told us that the mutant br gene was transformed into l33
Br基因的定点突变改造和突变基因在嗜盐菌中表达系统的建立使我们可以研究br的各种突变蛋白。 |
| 5. | Morphological studies indicated that most strains " taxonomy status were hard to determine only by morphological characteristics . most of the new isolations were moderately halophiles
研究发现，分离的放线菌菌株多数为中度嗜盐菌，生长最适盐度为5 - 10 。 |
| 6. | In order to explore halocin diversity at both the protein and gene expression levels , 22 haloarchaeal strains were used to study antagonistic interactions among them
对这6个嗜盐菌素分别进行理化性质的测定，发现halc8性质非常稳定，是研究嗜盐菌素的理想材料。 |
| 7. | Bacteriorhodopsin ( br ) is the sole protein present in the purple membrane of halobacterium halobium . the biological function of the br is acted as a light - driven proton pump
细菌视紫红质( bacteriorhodopsin ，简称br )是嗜盐菌细胞膜上的一种光敏蛋白质，具有光驱动质子泵功能。 |
| 8. | Qd3 . among them , six halocins ( hal c6 , hal c8 , hal c9 , hal c16 , hal cl7 and hal c22 ) displayed wide inhibitory spectra and could inhibit the growth of most ( > 10 ) haloarchaeal strains tested
纯化后的嗜盐菌素c8在tricinesds - page上表现为单一条带，分子量大小为6 . 3kda ，等电聚焦测得其等电点为4 . 4左右。 |
| 9. | These results revealed that halocin c8 is cytocidal and its primary target might be located in the cell wall of the sensitive cells . two degenerate oligoxynucleotide primers ( c8 - 2 , c8 - 4 ) were designed according to the n - terminal amino acid sequence of halocin c8
根据嗜盐菌素cs的n端氨基酸序列设计了一对简并引物( cs一2 ， cs礴) ，利用这对引物从as7002的总dna中扩增出halcs的部分序列。 |
| 10. | After absorption , the all - frans - retinal isomerizes to a 13 - c / s configuration and br undergoes a photocycle : br570 k590 l550 m410 n520 o640 br570 bacteriohodopsin is a promising biological photoelectric material . we intent to conduct a more thermostable br by site - directed mutagensis . a point ( no . 274 t , no . 274 a turn to c g ) mutation was introduced to br gene by successive pcr technique , and cloned into puc - 19 vector
本论文利用连续pcr的方法定点突变了br基因的一个氨基酸，突变位点选择了br基因第273的t和274位的a ，把它们变为cg ( tyr替换为arg ) ，然后把突变的br基因克隆入嗜盐菌表达载体pnov - r ，经测序鉴定的表达载体转化br缺陷的嗜盐菌，构建了tyr79 argbr突变体。 |
