| 1. | Construction of murine phage antibody library and selection of n - peptide - binding single - chain antibodies
小鼠噬菌体抗体库的构建和n -肽结合单链抗体的筛选 |
| 2. | Independent clones was constructed and affinity panning of anti - n - peptide antibody fragments was carried out
的小鼠噬菌体抗体库，并从中淘选出对n -肽有结合活性的单链抗体。 |
| 3. | Selection of anti - hcg scfv from murine phage antibody library selection of anti - hcg scfv from murine phage antibody library
从噬菌体抗体库中淘选人绒毛膜促性腺激素的单链抗体 |
| 4. | The phagemid particles displaying functional scfvs were rescued by reinfection of helper phage m13ko7 , thus a murine antibody library was obtained
经辅助噬菌体m13ko7超感染回收全部重组噬菌体，此即噬菌体抗体库。 |
| 5. | The murine phage antibody library was amplified and then panned by human chorionic gonadotropin for four rounds . the last round enriched phage clones were used to reinfect
由鼠源噬菌体抗体库淘选到展示有人绒毛膜促性腺激素单链抗体的噬菌体克隆。 |
| 6. | In the present paper the application of dna library , phage display random peptide library , phage antibody library , etc . in the research of serodiagnostic reagents and their special values were reviewed
本文综述了基因文库、噬菌体展示肽库及噬菌体抗体库技术在血清学诊断试剂研制中的应用及其各自的优点。 |
| 7. | The patterns of sds - page indicated more than 30 % of recombinant proteins could be obtained from the extract of e . coli bl21 . furthermore , library of recombinant phage antibodies was constructed from total rna isolated from spleen of the female balb / c mice immunized by bull sperm
首先用牛精子免疫雌性四周龄balb c小鼠，从其脾脏组织分离总rna ，应用重组噬菌体抗体库技术，构建了一个针对牛精子的噬菌体抗体文库。 |
| 8. | It has been demonstrated directly or indirectly - 7 - that ak auto ab is an important element in the immune network and plays a important role in maintaining physiological functions , clearing aged cells and metabolic products , regulating immune responses and protecting against infection . in some pathological states such as psoriasis and contact dermatitis , a certain serum level of the antibody could inhibit the progression of the diseases , and is beneficial to the recovery from the diseases . after a long time studies on the production and regulation mechanism of physiological and pathological auto antibodies , meanwhile , experiencing an intensive academic debating on whether naas a " horror autotoxicus " or a " gnothi seaution ( know yourself ) " , a common viewpoint has been achieved that naa is of clinical significance in the treatment of immunity diseases for it ' s function in the immune system stability , immunoglobulin y and polyclonal ak auto abs have been used in treating inflammatory dermatitis , and recombinant antibody is under investigating
抗角蛋白自身抗体( akautoab )是naa的重要组成部分，以往实验通过杂交瘤技术、免疫亲和层析技术和噬菌体抗体库技术分别获得单克隆akautoab 、健康人血清多克隆akautoab和基因工程人akautoab ，并对akautoab免疫学特性及在体生理和病理意义进行了广泛的研究，直接或间接地发现akautoab是机体正常免疫调节网络的组成部分，在维护某些生理状态的稳定、清理衰老细胞及代谢产物、调节免疫和抗感染等方面起到重要作用；在某些病理情况下(如银屑病、接触性皮炎等) ，体内akautoab的组分和滴度会发生变化，而正常水平的akautoab则有利于限制病情的发展，促进损伤的修复。 |
| 9. | Methods : a set of oligonucleotide primers were designed and used to amplify the vh and vl gene from anti - hbsag fab antibodies screened from phage antibody library . the products were cloned into puc19 vector and their sequences were analysed . the vh and vl gene fragments were tethered by a peptide linker and a leader sequence coding region , with the leader sequence added at 5 " terminus of each gene ( l - vh - linker - vl ) and designated as l - scfv
方法以从噬菌体抗体库中筛选获得的抗hbsag的fab抗体基因为模板，分别扩增出其轻、重链可变区( v _ l 、 v _ h )基因，通过重组pcr方法将轻、重链可变区基因用连接肽( gly _ 4ser ) _ 3的编码序列连接，并引入前导肽编码序列，构建具有l - v _ h - linker - v _ l结构的单链抗体基因。 |
| 10. | Panning and enrichment ofmg7 recombinant phage antibody the transformed cells were infected with m13k07 helper phage to produce a phage form of mgy recombinant phage antibody library ; after three rounds panning of the library with the mgrbinding antigen highly expressed gastric cancer cell line kato iii , the mg7 scfv displayed phage dones were selected from the enriched recombinant phages by elisa
3 mg _ 7重组噬菌体抗体库的淘筛用m13ko7辅助噬菌体感染转化菌，以挽救出噬菌体形式的抗体库；用高表达mg _ 7抗原的胃癌细胞系kato对抗体库进行三轮淘筛；用elisa筛检呈现有mg _ 7scfv的噬菌体单克隆。 |
