| 1. | More studies were conducted on both the physiology and ecology of the cyanophage
在上述研究的基础上，我们还系统开展了噬藻体生理与生态特性的研究。 |
| 2. | Investigation in details on a freshwater cyanophage isolated in china infecting plectonema and phormidium is presented in this thesis
我们在国内首次分离和报道了淡水噬藻体(蓝藻病毒) ，其宿主为织线藻和席藻。 |
| 3. | Under trasmission electronic microscope ( tem ) , the cyanophage showed a bacteria - phage shape with head diameter at 52nm and short tail ( almost invisible )
电镜照片显示该噬藻体为噬菌体形态，头部直径为52nm ，尾部很短，几乎不可见。 |
| 4. | Under the dark condition or at low temperatures , the cyanophage would n ' t multiply , but the elevation in both temperature and sunlight would bring about the burst size to increase
测定释放量和潜伏期的实验结果表明，温度和光照是主要的影响因子，在无光照或温度较低的情况下，噬藻体几乎不增殖。 |
| 5. | This method was also effective for concentrating virus form large volume water samples . moreover / the concertration multiple of cyanophage pp could rise to nearly one - hundred times when two - step backflushing ultrafiltration technique was adopted
此法也适用于大量水样的处理，而且当采用两步反冲超滤技术时，噬藻体的浓缩倍数可以提升到近100倍。 |
| 6. | Several methods for measuring the lysing cycle and burst size of the cyanophage were tested . it was established that cell counting method was more convenient and exact for confirming the lysing cycle . burst size obtained by 1 pfu infecting was reliable
我们研究了确定该噬藻体裂解周期和释放量的多种方法，认为细胞计数法是测量裂解周期既方便又准确的方法，而采用1pfu的感染可获得噬藻体的准确释放量。 |
| 7. | In this thesis , the fist cyanophage isolated in china was investigated with regard to the molecular biological characters . techniques for the large harvest and the electronic microscopy negative staining of the cyanophage were established . the genome library was constructed
论文对我国淡水中分离得到的一株蓝藻病毒(噬藻体)的某些分子生物学特性进行了研究，建立了其大量增殖与电镜染色的技术，构建了基因组文库，并对部分序列进行了测定与和分析比较。 |
| 8. | A new concentration system , the backflushing ultrafiltration system , was constructed with the isolated cyanophage pp in our lab associated with the ultrafiltration technique . basing on this system , the cyanophage concentration multiple could reach up to 30 times and the recovery rate of the cyanophage could exceed 90 %
浓缩是研究噬藻体的重要前期工作，我们以本实验室分离的淡水噬藻体pp为实验材料，结合超滤技术，建立了一套能够有效浓缩水样中噬藻体的方法- - -反冲超滤法。此浓缩技术可使噬藻体的回收率达90以上，浓缩倍数达30倍。 |
| 9. | Under mixed culturing conditions , it was observed that bacterial number rapidly incre ; ised soon after the lysing of host cells . on the contrary , while the non - host cyanobacterium ( i . e . anabaena flos - aquae ) was incubated in the mix culturing system , the breeding of the bacteria could be inhibited . it may be suggested from the result above that cyanophage could bring about the changes in microorganism populations
调查结果显示， 19个采集的水样中有6个含有裂解织线藻的噬藻体，而且水样未经浓缩即能裂解宿主，说明噬藻体在淡水中分布较广泛，裂解性也较强；混合培养条件下的研究结果表明，噬藻体裂解宿主后，细菌数量快速增加，而当培养系统中有非宿主藻类存在时，细菌的增殖则受到非宿主藻的抑制，说明噬藻体可以显著改变系统中微生物的种群结构。 |
| 10. | Again , using cyanophage pp and adopting orthogonal design , we have performed the studies on the effects of several environmental factors on the multiplication of the cyanophage . results are as follows : in the experiment of measuring the adsorption rate , temperature was found to be the primary influencing factor and displayed an obvious significance by the statistics analysis
以噬藻体pp为对象运用正交设计的实验方法，我们还研究了几种环境因子对噬藻体增殖的影响，结果如下：在测定吸附率的实验中，我们发现温度是主要的影响因素，并且在统计学上有显著意义。 |
