| 1. | Construction and filtration of the prokaryotic expression system of halr
人肝再生增强子原核表达系统的构建与筛选 |
| 2. | There is no difference in range of gus activities using constructions in which the activator elements are cloned in opposite orientation
据此提出启动子和增强子通过反式作用因子互作的机制模型。 |
| 3. | Odd or even copies of ocs activator follow the rule that addition of multiple ocs activators upon mas promoter increases promoter strength
研究发现正反向插入的同一拷贝ocs增强子作用相同，不受方向的限制。 |
| 4. | The upstream region - 419 - 90 of promoter camv35s was repeated 2 times manually , and then became enhancer e12which can improve promoting efficiency of promoter camvsss to 70 folds
增强子e12是将camv35s启动子上游- 419 - 90区域重复2次所构成的序列，该序列可使camv35s启动子在双子叶植物中的启动效率提高70倍。 |
| 5. | Of the combinations we tested , the construction containing four copies of ocs activator resulted in the highest level of expression , which strongly elevated the expression of gus activity in leaf ( 23 - fold ) and root ( 47 - fold ) compared with 35s promoter
其中4拷贝ocs增强子和mas启动子构建成的融合启动子活性最高，在叶中是camv35s启动子的47倍，在根中是23倍，增强效果显著。 |
| 6. | These results demonstrated the acbadh promoter is a strong salt - stress - induced promoter . the acbadh 5 ' - flanking region contains two salt - responsive enhancer regions localized between - 1114 and - 892 , - 464 and - 234 and one sliencer region localized between - 892 and - 643
对转基因烟草的gus活性分析表明，在1114和892之间， 464和234之间存在增强子元件，而在892和643之间存在负调控元件。 |
| 7. | However , even copies of ocs activator enhance promoter strength higher than odd copies of ocs activator . in order to explain results above we propose a model that multiple copies of activator interact with promoter via contacts between dna - bound transcription factors . 3
在叶中是奇数倍和偶数倍的ocs增强子分别随着拷贝数的增加而累积增加，且偶数倍的ocs增强子要比前一个奇数倍的增强子活性大。 |
| 8. | Ts87 gene fragment was picked out by screening adult t . s cdna library using sera of rabbit raised against soluble antigen of t . s and using sera of rabbit infected artificially with t . s . the ts87 fragment was ligated to vector pcdnas . l , which contains a cmv promoter / enhancer
本研究采用通过免疫筛库得到的旋毛虫抗原基因片段ts87与载体pcdna3 . 1连接。该载体含有人类巨细胞病毒( cmv )的高效启动子和增强子，是一种外源基因在哺乳动物细胞内高效表达的理想载体。 |
| 9. | Degenerate oligonucleotides to highly conserved regions of cucumis melo 1 - aminocyclopropane - 1 - carboxylic acid ( acc ) oxidase gene were used to prime the amplification of fragment of 128bp by ploymerase chain reaction ( pcr ) in samples of genomic dna from fruit of cucumis melo l . cv hetao flesh , which was cloned into plasmid vector pmd - 18 - t . the clon of antisense orientation were selected , and it was inserted downstream of camv35s promoter and enhancer " " of tmv into the plant expression vector pbinyxw , antisence expression vector pbinya was constructed . at the base that pollination and fertilization of cucumis melo l . cv hetao was studied , using pollen tube pathway transformate cucumis melo l . cv hetao , 76 fruit had been obtained , moreover , hardness and content of sugar were analysed
本实验以河套蜜瓜果肉基因组dna为模板，用甜瓜acc氧化酶基因特异寡核苷酸链为引物进行pcr扩增，得到128bp的扩增产物。将得到的扩增产物克隆到质粒载体pmd - 18 - t上，筛选反向克隆，然后将其反向构建到植物表达载体pbinyxw的camv35s启动子和tmv增强子“ ”的下游，构建成反义表达载体pbinya 。并在对河套蜜瓜授粉受精生物学研究的基础上，通过花粉管通道法转化河套蜜瓜，共获76颗瓜，并进行了硬度和含糖量的分析。 |
| 10. | Ln our experiment , a lot of regulator sequences inc1uding the 35s promoter with double enhancer e1ements and the terminator nos , the fl sequence and kozak sequence for improving the genetic statement in the level of translation , were appended on the two s ides of the artifically pseudopleuronectes americanus antifreeze protei n gene by a series of process , and the products were linked into pbil21
本实验中人工合成了美洲拟鲽抗冻蛋白基因，并在美洲拟鲽抗冻蛋白基因两侧添加了许多调控序列，如具有加倍增强子的35s启动子和nos终止子，它们可以促进抗冻蛋白基因的转录，及可在翻译水平上提高基因表达的序列和kozak序列，从而构建了抗冻蛋白基因的高效表达盒。 |
