Chinese translation for "多次传代"
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- high passage
Related Translations:
多次作案: repeatedly commit crimes 多次通过: multiple traversalmultiple-pass 多次覆盖: multifold coveragemultiple coveragemultiplecoverage 多次点火: multistage ignition 多次再生: multiple regeneration
- Example Sentences:
| 1. | In trpsin tolerance assay . this virus could resist to 1 % trpsis at 37 in an hour . in acid tolerance assay , this virus was resistant to ph3 . 0 and ph5 . 0 at 37 in 2 hours , and the average infection litre of the virus decreased little . in heat assay , at 50 , the virus was processed from 5 minutes to 150 minutes and at each condition the viral virulence reduced to some certain degree . among these conditions , when at 50 in 30 minutes . the average infection litre of this virus decreased over 2 tilre . and when al 50 in an hour , cpe of ihis virus disappeared . when time was set for an hour . but with processed in different temperature as 50 60 70 , 80 , the virus losl the multiplication capacity complelely . in biological assay , we selected different cell lines to cultivate this virus by laking advantage of possesional cells at that time in our laboratory . then we found that fcwf cell line was the most sensitive to dxmv and mdck was the second . with f81 cell line , after passaged for 12 times continuously with low concentration of fcs . the virus could produce cpe . however , with vero cell line . the virus could not procuce any cpe after many passages . the hemagglutination and lumadsorption reaction test proved that this virus had no any reaction to erythrocyte of pig , fowl and cavy . by neutrolizaion assay , dxmv could be identified as a kind of ccv 理化学研究表明,该病毒为rna病毒,对氯仿、乙醚敏感;胰酶试验中,经37 、 1小时处理的病毒,仍然能够在猫源细胞fcwf细胞上生长,并且毒力基本保持不变;耐酸性试验中,病毒分别在ph5 . 0和ph3 . 0经37作用2小时,毒力仅下降一个滴度;耐热性试验中,该病毒在恒定温度50 ,设定不同时间,从5分钟到150分钟,毒力均有不同程度下降,其中, 50作用30分钟,病毒平均滴度下降2个单位; 50 , 60分钟, cpe消失;恒定时间1小时,设定不同温度( 50 - 60 - 70 - 80 ) ,病毒在细胞上完全丧失增殖能力, cpe消失。生物学试验,利用实验室现有条件,选择不同的细胞系对该病毒进行培养,发现该病毒对猫源细胞fcwf最敏感; mdck细胞次之; f81细胞经多次传代,亦可出现cpe ;而vero细胞则不敏感。血凝试验表明,该病毒对猪、鸡、人及豚鼠的红细胞均无血凝性。 |
- Similar Words:
- "多次抽样检样" Chinese translation, "多次出入境签证" Chinese translation, "多次触球" Chinese translation, "多次穿孔" Chinese translation, "多次穿膜蛋白" Chinese translation, "多次淬火" Chinese translation, "多次存储卡" Chinese translation, "多次打回" Chinese translation, "多次打击" Chinese translation, "多次代换编码系统" Chinese translation
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