| 1. | Purification and identification of prothesia similis nuclear polyhedrosis virus
盗毒蛾核型多角体病毒的分离鉴定 |
| 2. | Effect of several conditions on propagation of mamestra brassicea nuclear polyhedrosis virus in vitro
某些培养条件对甘蓝夜蛾核型多角体病毒离体增殖的影响 |
| 3. | The 21kda protein was specifically located in the nucleocapsid of occlusion - derived and not in that of budded virions
进一步的研究显示， ha122蛋白特异的存在于多角体病毒核衣壳中。 |
| 4. | Here we report the identification of two of those , ha34 and ha99 , discussing in separate chapter ( chapter 3 and chapter 4 )
第二章对棉铃虫核多角体病毒( hasnpv )的一个未知功能基因ha34进行了鉴定。 |
| 5. | Chapter two is about the sequence analysis of the xbai - i fragment of helicoverpa armigera single - nucleocapsid nucleopolyhedrovirus . the xba - fragment located at 18 . 4 - 22 . 8 m . u
第二章报道了棉铃虫单核衣壳核多角体病毒( hasnpv ) xba -片段的序列结构。 |
| 6. | Chapter two is a report on genetic organization of the hindlll - l fragment of hasnpv . the fragment that is 7501 bp long and located at map units ( mu ) 12 . 3 - 17 . 9 of the viral genome was sequenced
对棉铃虫单核衣壳核多角体病毒( hasnpv )的研究历史和近期对hasnpv的基因组学研究以及本论文的研究内容作了简要介绍。 |
| 7. | At the time of larvae number peak , some biological pesticides were adopted to control in the proper time , which might reached the aim of controlling the damage of the above insect , and owned the environment protected effects
在幼虫数量高峰时，适期采用苏云金杆菌、棉铃虫多角体病毒、阿维菌素等生物农药（制剂）进行防治，可达到控制该虫为害的目的，且具有环保效应。 |
| 8. | The homology of the other two motifs ( v and vi ) , which are also quite conserved in other helicases , were lower than 30 % . the hasn pv helicase protein had considerable amino acid sequence similarity with other baulovirus helicases ( 58 % ) , and the highest ( 66 % ) with semnpv and the lowest with xcgv . ( 43 % ) . hasnpv helicase was the first helicase reported in single - nucleocapsid nucleopolyhedrovirus there are 5 homologue regions ( hrs ) in hasnpv genome which may play important roles in the viral replication
同源性比较发现hasnpv解螺旋酶的氨基酸序列与甜菜夜蛾核多角体病毒( spodopteraexiguemnpv ， semnpv )的解螺旋酶具有最高的同源性( 66 ) ，与xestiac - nigrum颗粒体病毒( xcgv )解螺旋酶同源性最低( 43 ) ， hasnpv解螺旋酶基因是第一个报道的单粒包埋核多角体病毒的解螺旋酶基因。 |
| 9. | In the experiments discussed in chapter 5 we generated two recombinant viruses based on an acmnpv - and hasnpv - bac - to - bac system , respectively . in such recombinant viruses the busuctl gene under polyhedrin promoter was inserted into polyhedrin gene locus . a preliminary bioassay was conducted
第五章利用杆状病毒bac - to - bac系统构建了含有油桐尺蠖核多角体病毒的类蜗牛毒素基因的重组病毒racbacctl和rhabacctl ，在其相应宿主甜菜夜蛾和棉铃虫的细胞水平和虫体水平进行了超表达实验。 |
| 10. | In order to investigate the genomic organization of the single - nucleocapid nucleopolyhedrovirus of helicoverpa armigera , the ecori - n fragment located at 54 . 8 - 59 . 3 kbp of the viral genome was sequenced . the fragment contained 3762 bp helicase gene potentially encoding a protein with a molecular mass of 146 kda
对棉铃虫单核衣壳核多角体病毒( helicoverpaarmigerdsingle - nucleocapsidnucleopolyhedrovirus ， hasnpv )基因组中ecori ? n片段进行序列分析，获得了完整的解螺旋酶基因( hel ) ，其开放阅读框大小为3762bp ，编码一个分子量为146kda的蛋白质。 |
