| 1. | Studies on quantum dots - labeled trichosanthin
量子点标记天花粉蛋白的研究 |
| 2. | High concentration of ca2 + also cannot affect the structure of tcs
高浓度的ca ‘ “也没有影响天花粉蛋白的内源荧光。 |
| 3. | Recent studies indicated that tcs has curative effect for the treatment of cancer , aids etc . therefore , studies on tcs are of important values in both academic research and practical application
近几年的研究表明，天花粉蛋白对癌症、 aids等疾病有一定疗效。天花粉蛋白也是一种金属蛋白。 ca ~ ( 2 + )处于其活性中心部位。 |
| 4. | In conclusion , the five metal ions examined do not shift the maximum fluorescent emission at 332nm , while cu2 + and fe3 + can disturb the structure of tcs and perhaps affect the function of protein in some extent
试验也研究了天花粉蛋白与edta的相互作用，发现当edta浓度达到40mmol / l (是反应体系中蛋白浓度的2万倍)时， edta对天花粉蛋白内源荧光强度的影响依然很微弱。 |
| 5. | Trichosanthin ( tcs ) is a single - chain ribosome inactivating protein . tcs belongs to a sort of metalloprotein , and ca2 + locates in its active center . it is isolated from trichosanthes kirilowii tubers of cucurbitaceae and is the active component of chinese drug tian hua fen
天花粉蛋白是从葫芦科植物栝楼块根中分离得到的一种单链核糖体失活蛋白，是中药天花粉的有效成分，具有重要的理论研究和实际应用价值。 |
| 6. | Though zn 2 + and co 2 + are divalent ions , they probably can not substitute ca2 + from the active center of tcs , or can substitute ca2 + but does not change the structure of tcs . there is no significant change observed for the fluorescence intensity of tcs
‘ “是二价金属离子，但这两种离子与天花粉蛋白作用时可能并没有取代天花粉蛋白活性部位的ca ‘ ” ，或部分取代但并没有改变天花粉蛋白分子的空间结构，以致天花粉蛋白的荧光强度无明显变化。 |
| 7. | The morphology of single cell and the ultrastructure of cell membrane were observed . by means of afm , the ultra - thin sections of murine es cells were investigated in order to make afm capable of gaining the information of the inner structure of cells . in addition , the morphological changes and damaging effect of trichosanthin ( tcs ) on red blood cell ( rbc ) membrane were observed by afm
对原子力显微镜( atomicforcemicroscope ， afm )的成像技术进行了多方面探索；用afm研究胶原蛋白分子在云母表面的吸附和自组装行为；对小鼠胚胎干细胞和人血红细胞进行afm成像，观测单个细胞的形态以及细胞膜的微观结构；利用afm得到了小鼠胚胎干细胞超薄切片的高分辨率图像，探索用afm研究细胞内部结构，拓展其应用领域；天花粉蛋白( tcs )和红细胞的相互作用，利用afm观察到天花粉蛋白( tcs )和红细胞相互作用前后红细胞膜超微结构的变化，据此讨论了二者的作用机理。 |
| 8. | Red blood cells were imged by afm and the typical biconcave shapes were observed . the ultrastructure of rbc membrane was also gained . the morphological changes of red blood cell ( rbc ) membrane after the interaction of trichosanthin ( tcs ) were clearly observed by afm
对红细胞进行成像，观察到红细胞双面凹的典型特征，小范围扫描观察到了红细胞膜表面的超微结构，在此基础上研究了红细胞与天花粉蛋白作用后红细胞膜表面超微结构的变化，并对二者作用的机理进行了分析讨论。 |
