| 1. | Studies on enhaning effect of alfalfa polysaccharide on the immune response of lapinized chinese strain of classical swine fever virus
苜蓿多糖对猪瘟兔化弱毒疫苗免疫应答强化作用的研究 |
| 2. | The sows , which are not pregnant , are vaccinated by hcv vaccine of different dose and then detected antibody against hcv with ppa - elisa at different time
给空怀母猪接种不同剂量的猪瘟兔化弱毒疫苗，分别于不同时期用ppa - elisa检测血清抗体。 |
| 3. | Currently , fmd vaccines are either conventionally inactivated vaccines or attenuated vaccines . both types of vaccines are not completely safe and pose some fatality risks
而传统的灭活疫苗和弱毒疫苗由于其潜在的危险性，使其应用受到很大的限制。 |
| 4. | The chinese donkey leukocyte attenuated strain of eiav （ dla ） was the only successful lentivirus vaccine up to now , which has been applied extensively in china against eia
我国的eiav弱毒疫苗是迄今为止世界上唯一投入应用的慢病毒疫苗，成功地控制了我国eia的流行。 |
| 5. | Hc ( hog cholera ) , the virulent infectious disease caused by hcv ( hog cholera virus ) , had been controlled effectively by hclv strain vaccine in the country
猪瘟是由猪瘟病毒引起的猪的一种病毒性传染病。猪瘟兔化弱毒疫苗有效地控制了猪瘟在我国的急性发生和大流行。 |
| 6. | Up to now , there are still some infectious diseases that cannot be prevented effectively . the traditional vaccines are mostly less safe or efficacious , driving efforts to develop vaccines of new generation
迄今为止，许多传染性疾病尚未得到有效防治，现行的灭活疫苗或弱毒疫苗等传统疫苗或免疫效力低下，或存在安全性问题，促使人们开发安全有效的新型疫苗。 |
| 7. | In this study , a 1 . 7kb kpni fragment and a lacz gene expression cassette carrying the e . coli lacz gene under the control of sv40 promoter were inserted into the transfer vector pbdtk - uni ( a 277bp acci - accl fragment in the tk gene was deleted ) . the new transfer vector was called puni - lacz . the transfer vector puni - lacz and purified genomic dna of strain bartha - k61 were used to cotransfect vero cells using lipofectin transfection procedure
本研究以呈ge ~ -表型的经典弱毒疫苗bartha - k61株为亲本株，在通用prv转移载体pbdtk - uni的基础上，在其多克隆位点中插入由sv40早期启动子控制下的lacz基因表达盒，同时将下游同源臂增加了一个1 . 7kb的kpni片段，使上下游同源臂的长度都超过了1kb ，构建了一个新的转移载体puni - lacz 。 |
| 8. | It has been reported that the eiav s2 is not essential and does not appear to affect virus infection and replication in vitro . thus , we introduced a his - tag into the s2 gene of an eiav infectious molecular clone recombinant plasmid ( pok8266 ) by using soe pcr method and obtained a new recombinant plasmid with his - tag , designated as eiav - pok8 . 2 - his
本研究应用已构建好的eiav驴白细胞弱毒疫苗株的感染性分子克隆载体（ pok8266 ）为模板，通过soepcr方法在感染性分子克隆载体的s2基因独特区内引入突变点，形成含有酶切位点（ nspv ）的突变体（ p1p4 ） 。 |
| 9. | The comparative analysis of sequences indicated that the sequences of meq in different pathotypes are relatively conserved and the homology of the amino acid sequences is very high . the significant differences include two mutations in both mdv - 1 vaccine strains cvi988 / rispens and 814 strain : the deletion of a proline ( no . l93aa ) , and this mutation is just exactly located in a 15 - amino - acid ( eelcaqlcstppppi ) repeat sequence within the c - terminal transactivation domain of meq protein ; and a point mutation with a shift from alanine ( a ) of all virulent strains to serine ( s ) was occurred on the no . 71 aa
结果发现， mdv不同致病型的meq基因序列相对比较保守，它们相互间核苷酸和氨基酸序列的同源性均很高；但是，与所有七个致瘤性的mdv毒株相比，二个型弱毒疫苗cv1988 rispens株和814株均出现了两个特征性的突变：即第194位的p缺失性突变和第71位氨基酸由a变成了s的位点突变；缺失性突变恰恰位于meq基因中转录激活域内的一个多脯氨酸的重复序列( pppp )之中。 |
| 10. | The study is mainly about finding the reason that caused atypical hc through analysis and homology comparison on e2 gene sequences and amino acid sequences of e2 glycoprotein among strains hclv , fc , hcv - jn , hcv - yc , shimen et . al . at last , the best program against hc was established in order to control and erase hc
本研究主要是对猪瘟兔化弱毒疫苗、猪瘟病毒野毒株抗原基因( e2 )进行序列分析和同源性比较，分析发生非典型性猪瘟的原因，并在此基础上制定最佳猪瘟免疫程序。 |
