| 1. | 10 clones resistant to spectinomycin were obtained , 3 resistant transformants were subjected to three rounds of spc selection
其中挑取3个抗性转化子进行三轮的抗生素同质化筛选。 |
| 2. | Take a portion of the same colonies as under 3 . 8 . 1 by means of a wire and stab inoculate a glucose agar tube . incubate at 37 ? 1 c for 24 h
用接种针挑取与3 . 8 . 1实验相同的菌落的一部分接种至葡萄糖琼脂试管. 37 ? 1 c培养24小时。 |
| 3. | But of their children , who were left after them in the land , whom the children of israel consumed not , them did solomon make to pay tribute until this day
8就是以色列人未曾灭绝的，所罗门挑取他们的后裔作服苦的奴仆，直到今日。 |
| 4. | The asd mutants of salmonella thphimurium have an obligate requirement for diaminapimlic acid ( dap ) and will undergo lysis in environments deprived of dap
挑取单菌落质粒鉴定后，分别将其电击经中间宿主x3730转入最终宿主x4072 。 |
| 5. | Here is the account of the forced labor king solomon conscripted to build the lord ' s temple , his own palace , the supporting terraces , the wall of jerusalem , and hazor , megiddo and gezer
15所罗门王挑取服苦的人、是为建造耶和华的殿、自己的宫、米罗、耶路撒冷的城墙、夏琐、米吉多、并基色。 |
| 6. | And this is the reason of the levy which king solomon raised ; for to build the house of the lord , and his own house , and millo , and the wall of jerusalem , and hazor , and megiddo , and gezer
王上9 : 15所罗门王挑取服苦的人、是为建造耶和华的殿、自己的宫、米罗、耶路撒冷的城墙、夏琐、米吉多、并基色。 |
| 7. | And this is the reason of the levy which king solomon raised ； for to build the house of the lord ， and his own house ， and millo ， and the wall of jerusalem ， and hazor ， and megiddo ， and gezer
15所罗门王挑取服苦的人，是为建造耶和华的殿，自己的宫，米罗，耶路撒冷的城墙，夏琐，米吉多，并基色。 |
| 8. | The amplified phage be used repeat the selection . the selection was repeat three times . the titter of phage indicated that the eluted phage enriched with the rounds increasing
山西医科大学硕士学位论文第三次淘洗的噬菌体感染大肠杆菌xli一blue ，铺制平板，随机挑取18个分离良好的噬斑，制备噬菌体原种。 |
| 9. | 3 . the cold - heat method without the snailase and zymolyase was used as the pcr direct selecting of colony . to select the colony and suspend with 10 n 1 ddw , and then incubate
改进了重织酵母囱落的pcr鉴定方法：冷热处理法，即挑取单克隆于10ul水中恳浮， m及5分钟、液氮处理2分钟， 9了c15分钟，然后进行kr扩增。 |
| 10. | Cells were then harvested by centrifugation and the pellet was resuspended in phosphate - buffered saline ( pbs ) containing 5 mm edta . after sonication , debris was removed by centrifugation at 10000 x g for 15 min at 4
挑取转化后的大肠杆菌提取质粒， ecori和hindln酶切质粒进行鉴定，琼脂搪电泳显示含有大约800kb的目的片段。 |
