| 1. | It was also proved that the biosynthestic genes of apramycin was linked to the apramycin resistant gene in s . tenebrarius
Pcr实验证明基因重组菌株中接合转移质粒同源整合到黑暗链霉菌h6的染色体上。 |
| 2. | The gene recombinant strain no . 42 ca n ' t generate ampramycin , which indicated that the cloned gene is involved in apramycin biosynthesis in s . tenebrarius
通过接合转移的方法将质粒pzxb014导入黑暗链霉菌h6中，筛选基因发生重组的菌株。 |
| 3. | This system included method of solid medium plate enveloped with parafilm to form colony , conjugational gene transfer in a selective medium , screening the nonmagnetic mutants by magnet adsorption technique
此体系包括：以平板封膜培养技术获得单菌落，在选择性培养液中进行遗传因子接合转移，以液体培养和磁铁吸附技术筛选突变子。 |
| 4. | In order to facilitate the study of biological function of add gene cluster , e . coli - s . avermitilis intragenus conjugation system was established . in addition , phz2114 for the replacement of the entire add gene cluster and phz2130 for disruption of adda were constructed
建立了除虫链霉菌的接合转移系统，并构建了用于置换全部基因簇的基因置换质粒phz2114和adda的基因中断质粒phz2130 ，为研究除虫链霉菌add基因簇的生物学功能奠定了基础。 |
| 5. | Pfge analysis of these 21 blocked strains revealed a common chromosomal deletion in the 300kb asel fragment which might be responsible for antibiotic 5102 - iii biosynthesis . so the 300kb fragment was recovered and used as probe to hybridize with 10 - 22 genomic library . cosmids in this region were aligned and suitable fragments in this region were selected and used further for the construction of gene replacement plasmids
以此片段为探针钓出了位于该区域的文库克隆，并利用指纹印迹和杂交技术将这些文库克隆排列起来，进而以位于该区域的不同位置的片段做臂构建了用于转化和接合转移用的基因置换质粒，并试图通过基因置换将该区域置换下来，但尚未得到最终结果。 |
| 6. | A 1 . 5 kb apramycin resistance fragment was inserted into nru i site of aved gene and the inactivated aved gene fragment was then introduced into mcs region of phjl401 - an e . coli / streptomyces shuttle vector with conjugation function ( containing orit gene ) . as a result of above procedures , a recombinant plasmid pid03 was obtained
将1 . 5kb的安普霉素抗性基因片段插入到aved基因中的nrui酶切位点，再将此灭活的aved基因片段插入到具有接合转移功能(含有orit基因)的链霉菌?大肠杆菌穿梭质粒phjl401的多克隆位点区，由此得到重组质粒pid03 。 |
| 7. | Based on a 3 . 1kb pst i fragment of genomic dna of a wild s . avermitilis , a 1 . 5 kb apramycin resistance fragment was inserted into sph i site of avec gene in the 3 . 1 kb fragment , then a recombinant plasmid pc05 was obtained by introducing above inactivated avec fragment into mcs region of phjl401 . competent cells of et12567 were transformed by recombinant plasmid pid03 and pc05 respectively
以含有avec基因的3 . 1kb基因组dnapsti片段为基础，将1 . 5kb的安普霉素抗性基因片段插入到avec基因中的sphi酶切位点，再将此插入失活的avec基因片段连接到具有接合转移功能(含有orit基因)的链霉菌-大肠杆菌穿梭质粒phjl401的多克隆位点区，由此得到重组质粒pc05 。 |
| 8. | A fragment , containing 2 . 0kb cloned 5 . tenebrarius dna and reported genes of erme and xyle , was inserted in plasmid phz132 ( an e . coli - streptomyces shuttle plasmid incorporating orit from rk2 ) to construct disruption plasmid pzxb0l4 . the plasmid was transformed into e . coli et12567 ( puz8002 ) to construct recombinant e . coli et12867 ( puz8002 , pzxb014 )
将克隆到的链霉菌dna2 . 0kb片段以及报告基因erme 、 xyle插入到具有orit的大肠杆菌?链霉菌穿梭质粒phz132中构建接合转移质粒pzxb014 ，并将其转入大肠杆菌et12567 ( puz8002 )中，获得供体菌et12567 ( puz8002 ， pzxb014 ) 。 |
| 9. | In this study , the suitable parameters for the introduction of plasmids phz1358 and pset152 into s . nanchangensis ns3226 from e . coli were tested for developing an conjugation system for s . nanchangensis ns3226 . a dnd gene cluster , which encodes an unknown modification system for 5 hvidans 1326 and renders its dna susceptible to site - specific double - strand dna cleavage during electrophoresis was conjugated from e . coli into s . nanchangensis ns3226
将克隆在整合型载体pset152上的变铅青链霉菌1326的dnd基因簇通过接合转移导入野生型南昌链霉菌ns3226中进行异源表达，观察到接合转移子的dna获得了在含fe ~ ( 2 + )的电泳缓冲液中电泳时降解的表型。 |
