| 1. | Detection and bio - assay of insecticidal crystal protein bmb20 - 4 from bacillus thuringiesis
4的杀虫晶体蛋白检测及毒力测定 |
| 2. | Analysis of insecticidal crystal protein and its cry - type genes of bacillus thuringiensis isolates from china
分离株杀虫晶体蛋白及基因分析 |
| 3. | These insecticidal proteins are commonly known as insecticidal crystal proteins ( icp ) or delta endotoxin
这些杀虫蛋白通称为杀虫晶体蛋白（ icp ）或-蛋白。 |
| 4. | Advances in the rfsearch on crystalline inclusion proteins produced by symbiotic bacteria associated with entomopathogenic nematodes xenorhabdus and photorhabdus
昆虫病原线虫共生细菌胞内晶体蛋白的研究进展 |
| 5. | Two main parts were included in the present thesis . part i . cloning and expression cryigenes of bacillus thuringiensis in different host strains
一、苏云金芽胞杆菌杀虫晶体蛋白基因在不同受体菌中的表达1 |
| 6. | Most susceptible insects are killed by ingestion of the crystals alone ; a mixture of spores and crystals are required for a toxic effect in only a small number of insects
大多数易感虫种只要摄取晶体蛋白即可被杀死；只有一小部分昆虫要求孢子和晶体同时存在。 |
| 7. | 3 . construction of fusion genes with insecticidal protein gene and gfp the fusion genes was constructed by pcr . the pesticidal crystal protein gene crylac10 and gfp were chosen to construct the fusion genes
建杀虫晶体基因与gfp基因的融合基因pcr扩增全长gfp片段融合于去掉终止结构的杀虫晶体蛋白基因的c -端，构建融合基因。 |
| 8. | The results of sequencing and structural analysis showed that the gene , named crylaa14 by international nomenclature committee of bt 5 - endotoxin , was 3552 bp and the open reading frame encoded a 133 . 7 kda protein with 1183 amino acids
序列测定结果表明该基因编码区为3552bps ，编码1183个氨基酸，分子量为133 . 7kda ，等电点为pi4 . 755 。该基因序列已在genbank注册， accessionnumber为ay197341 ，并被国际bt杀虫晶体蛋白基因命名委员会正式命名为cry1aa14 。 |
| 9. | The research results are summarized as following : 1 . the relationship between gene types and the toxicity against s . exigua in order to construct satisfactory genetically engineered strain , it is first necessary to understand the relationship between gene types and the toxicity . nine strains containing crylc were chosen for study for this purpose
研究结果总结如下： 1菌株对甜菜夜蛾的毒力与基因类型的关系pcr扩增检测了九个苏云金芽胞杆菌菌株所含杀虫晶体蛋白( insecticidalcrystalprotein , icp )基因，根据扩增结果将它们分为5种基因类型。 |
| 10. | The recombinant plasmids pbmb121l , pbmb9821l and pbmb986l were constructed after transferring bacillus thuringiensis icps gene crylaal , crylaclo and crylca from their original plasmid vectors to different plasmid vector . the relevant recombinant strains were obtained after introducing the 3 recombinant plasmids into bacillus thuringiensis plasmid - free mutant strain 8mb 171 by electroporation . the transformation and expression properties of 8mb 171 were studied
通过将苏云金芽胞杆菌杀虫晶体蛋白基因cry1aal 、 cry1ac10和cry1ca转移至不同质粒载体上，分别构建了重组质粒pbmb121l 、 pbmb9821l和pbmb986l ，并分别导入无质粒突变株bmb171 ，筛选得到携带相应icps基因的重组菌株。 |
