| 1. | Identification of znf219 nuclear localization signals
219基因核定位信号序列的确定 |
| 2. | Both map2 and sap2 have two typical ap2 domains and a putative nuclear location signal , kksr
Map2和sap2内部都含有两个典型的ap2结构域，并且n端都具有高度保守的核定位信号。 |
| 3. | Similar to ap2 in arabidopsis , hap2 have a highly basic 10 - amino acid domain that includes a putative nuclear located sequence kksr
类似于拟南芥的a类基因apz ， hapz亦含有10个氨基酸长的高度碱性的区域，该区域具有核定位信号kksr 。 |
| 4. | At the same time , the c - terminal expressing plasmids of hcap - e and hcap - c that attached the kozac sequence and the nuclear localization signal were also constructed
同时也构建了附加kozac序列和核定位信号的hcap - e和hcap - c的c -末端真核表达质粒。 |
| 5. | To identify the transfected cells , we also constructed the gfp expressing plasmids which also attached the kozac sequence and nuclear localization signal to the gfp gene and the plasmids served as a selective marker for the transfected cells
为了区分被转染的细胞，以附加kozac序列和核定位信号的gfp ( pcdna3 . 1 + kg )作为被转染的细胞的标记。 |
| 6. | 3 ) the 3rd group of mutant alleles included f66 , f15 - 28 , f126 and f107 . these alleles showed wing and cuticle phenotypes related to wg signaling pathway . this group of mutant alleles turned out to be a new component of wg singaling pathway
这组的突变体等位基因编码一个wg信号传导途径组成成分干gg由其特点是在n端含有一个核定位信号mls ） ，而在c端则含有一个pho结构域（一种c4hc3锌指结构） 。 |
| 7. | In our studies , we have used the nuclear localization signal department of medical genetics and development biology 4 ( nls ) selection system to isolate a novel gene from a mouse embryonic cdna library . the full - length cdna is about 1802bp and contains an open reading frame of 1296 nucleotides . it encodes 431 amino acid
本研究工作，利用本室构建的核定位信号筛选系统从小鼠胚胎cdna文库中克隆到一个新的全长cdna片段，分析表明在其1802bp育回军区大学刃士学位论文的序列中含有一个长1293hp的开放阅读框，编码431个氨基酸。 |
| 8. | The effectiveness of the nuclear localization signal selected was clearly tested in initial experiments . based on this , the co - transfection systems used in the study was established . we first observed the multinucleate cells and chromatin bridges in cultured hela cells when the cells are marked with gfp and hochest33342 , after co - transfection with the gfp expression plasmids and rnai plasmids rhe or rhc
当分别共转染附加kozac序列和核定位信号的gfp与人集缩素smc亚基hcap一e特异的rnai质粒rhe和人集缩素smc亚基hca尸一c的f { nai质粒rhc时，都观察到gfp和hochest33342标记的转染后hela细胞表现多核和染色质桥现象。 |
