| 1. | Clinical epidemiological investigation on
毒力岛的临床流行病学调查 |
| 2. | Detection of related genes of
毒力岛相关基因的检测 |
| 3. | Schubert et al reported that hpi was found in 93 % eaggec , 27 % enteroinvasive e . coli , 5 % enteropathogenic e . coli and enterotoxigenic e . coli
在不同类致泻性大肠杆菌中， hpi毒力岛在eaggec中高频分布的原因，目前也不清楚。 |
| 4. | Virulence genes of pathogenic bacteria can be located on the chromosome where they may be organized as so - called pathogenicity island ( pai )
细菌毒力岛( pathogenicityisland ， pai )是指染色体上成簇排列的与细菌毒力密切相关的基因簇。 |
| 5. | Pai is not only attributed to the pathogenicity of bacteria and specific phases of the infection progress but also play an important role in the evolution of bacterium
毒力岛不仅赋予病原体特殊的致病能力，介导感染过程的特殊阶段，而且在细菌进化过程中扮演重要角色。 |
| 6. | The results show that there are 7 strains hpi positive except 1 strain hpi negative . hpi in 6 strains of 7 hpi - positive strains is inserted into asnt - trna site . the expression of fyua gene , which encoded fyua , the receptor of ybt , was upregulated by extracellular ybt level
研究结果提示，除1株eaggec中国分离株hpi ”外，其余7株均为hpi且7株中有6株携带的hpi毒力岛均插入在染色体的asnttrna位点。 |
| 7. | The reason that different distribution frequency of hpi in various e . coli is unclear , either . as the high frequency distribution of hpi in eaggec , this study will focus on the following parts to discuss the function of yersiniae high - pathogenicity island in enteroaggregative e . coli
鉴于hpi在eaggec中的高频分布，本研究拟从eaggec菌株出发，着重从以下几个方面对eaggec携带的hpi毒力岛的功能进行了研究。 |
| 8. | Supernatants of eaggec grown under normal condition ( lb medium ) and iron deficiency condition ( led medium ) were applied to the reporter strain , wa - cs irpl : : kn ( pcjg3 . 3n ) , which was transferred the reporter plasmid pcjg3 . 3n contained promoter region of fyua gene fused to gfp
Hpi毒力岛的力ua基因编码ybt受体fylla ，该基因的表达受细胞外ybt水平的正向调节，本研究利用丹ua基回启动子融合表达报告质粒pcjg3 3n及菌株wa csirpl ： kn （ pcjg3 |
| 9. | The pathogenic strains of yersinia enterocolitica carry a 45 - kb high pathogenicity island ( hpi ) comprising iron - uptake - related genes such as irp1 - irp2 and fyua , which has been observed in 93 % of the 60 entero - adhesive e . coli { eaec } strains and 80 % of the e . coli isolated from blood samples
Hpi毒力岛功能核心区主要的结构基因为irp2 、 irp1和fyua ，是高度保守的。 irp2和irp1分别编码两种高分子量蛋白hmwp2和hmwp1 ， fyua编码鼠疫菌素受体。 |
| 10. | The results showed that the f fragment , 728bp in length , could be a new gene with a little homology to the genes coding for polyketide synthetase or fatty - acid synthetase and the b fragment , about 4kb in length , is inferred to have repeat sequences around tn5 insertion site , in which there is homology to the wa 314 right arm of the high - pathogeniciry island of yersinia enterocolitica . to reveal any pathogenicity of enterobacter cloacae b8 and its mutated strains b8b and b8f to animals , the experiment with mice was carried out
结果显示， f片段长度为728bp ，与现有生物数据库的blast比较分析，发现该序列仅有局部短于1oobp的区域与polyketide合成酶基因或与脂肪酸合成酶基因有低的同源性，推测为一新基因； b片段长约4kb ，序列拼接结果推测靠近tn5插入位点部位有重复序列，对b片段tn5远端的部分序列进行blast比较，发现它与小肠结肠炎耶尔森氏菌的强毒力岛有一定的同源性。 |
