| 1. | This article dealt with cloning and sequencing of chitinase and endoglucanase genes of bacillus spp . and recombinant biocontrol isolates of bacillus spp
质粒分析证明克隆子中含有重组质粒，外源插入片段大小为2 . 6kb左右，与pcr最初产物的大小一致。 |
| 2. | Meanwhile , because the fragment of ctla4 extracellular domain is only 400bp , its expression will be more stable than that of ctlaig fusion protein ( 1 . 2kb )
同时，由于ctla4胞外区片段大小仅有400hp ，较ctla4ig融合蛋白zkb ）在表达菌中的表达将更为稳定。 |
| 3. | We designed double special primers for a rt - pcr to detect our results , and the result was similar to dd - pcr . then we detected all tissues using rt - pcr and found that rnf - 11 gene express highly in normal tissue and ctc - 50dm5 be in contract
差异cdna片段的验证rt一pcr反应结果显示两个反应的主带分别为500bp和450bp左右，符合引物设计中的扩增片段大小，与mrna - dd结果相符合。 |
| 4. | Sequence alignment shows that the fragment of pcr product showed some identities to the masp gene of xenopus laevis , branchiostoma belcheri , the trypsin gene in litopenaeus vannamei and the serine protease gene in aurelia aurita
Pcr反应产物基因片段大小为630bp ，序列比较结果表明， pcr克隆产物与爪蟾、文昌鱼的masp基因，虾、水母等的胰蛋白酶基因和丝氨酸蛋白酶基因都有一定的同源性。 |
| 5. | Discussion in the process of development , differentiation and metabolism , the differential expression of genes plays a pivotal role . mrna differential display , established by liang and pardee in 1992 , is among the most sensitive methods for the isolation of differentially regulated mrna
经rt一pcr反应筛查17例脊索瘤及其周围正常组织，扩增片段大小与引物设计一致，可见瘤周组织rnf一n基因表达明显增高，而ctc一sodms在肿瘤组织中表达增高。 |
| 6. | The main results are shown as below : 1 . 80 individuals from 4 populations ( 20 from each population ) of argopecten irradians were analyzed by using 20 random rapd primers . 132 rapd bands ranging from 230 to 2800bp were recorded with an average of 6 . 6 bands gained by per primer
采用20条随机引物对4个群体80个个体（每个群体20个个体）进行了rapd群体遗传多样性分析，共扩增出132个位点，片段大小在230 - 2800bp之间，平均每条引物的扩增带数是6 . 6条。 |
| 7. | Phaa , phab and phac were amplifed from the subclone of pseudomanas sp . producing pha by pcr . the gel electrophoresis analysis showed that the molecular weights of cloned phaa , phab and phac were equal to fragment speculated from three orfs
利用所提取的开放阅读框架的序列设计三对引物，采用pcr技术，从合成pha的亚克隆片段中分离出phaa 、 phab和phac三个基因片段，经凝胶电泳分析表明，所克隆的三个基因分子量大小与推测的三个开放阅读框架中基因片段大小一致。 |
| 8. | Two primers , designed according to the conserved regions of ban gene in arabidopsis thaliana , were used to amplify the ban homologous fragments from the genomic dna of brassica napus , b . chinensisl , b . juncea , a . thaliana and other cultural plants of cruciferae . the very similar pcr fragments were obtained from all the amplifications , which implicated that ban may be a conserved gene existing widely in the genomes of cruciferae . pcr fragments were cloned and confirmed by restriction enzyme digestion
参照拟南芥( arabidopsisthaliana ) ban基因与cdna设计引物，对甘蓝型、白菜型、芥菜型油菜，拟南芥及其它十字花科栽培品种的基因组dna进行pcr扩增，均扩增出与拟南芥ban基因扩增片段大小极其相似的dna片段，提示ban可能广泛存在于十字花科植物中。 |
| 9. | The library consisted of 1 . 3 x 106 clones with an average insert size of about 18kb . the capacity of this library was about 20 times the equivalent of the genome of atriplex centralasiatica iljin . screening the genomic library with a 400bp probe located at the 5 ' end of the badh gene obtained by rt - pcr , we got four positive clones
3x10 ‘个重组噬菌体，插入片段大小约为18kb ，含插入片段的频率为100队以中亚滨蓉甜菜碱醛脱氢酶门adh ）基因近5 ’端的约400hp片段为探针，筛选中亚滨蓉基因组文库，得到了4个阳性克隆。 |
| 10. | Total rna was extracted from pituitary of new butchered female goat for fsh - a and b subunit genes . cdna were synthesized by rt - pcr reactions respectively and these cdna were used as templates in pcr amplifications . the pcr products were 360bp and 390bp which just were the same length of the predicted goat fsh - a and p subunit genes
本试验是从新屠宰的母山羊脑垂体中提取总rna ，反转录获得cdna ，以此cdna为模板， pcr扩增目的基因片段，分别获得长为360bp的山羊fsh -亚基dna片段和长为390bp的山羊fsh -亚基dna片段，与预期的目的基因片段大小一致。 |
