| 1. | Screening the peptides specifically binding the injured endothelial cell with two phage - displayed random peptide libraries
噬菌体随机肽库中与受损内皮细胞特异性结合短肽的筛选 |
| 2. | ( 3 ) after one ptat - ha - scfv was expressed in e . coli bl21 , the product was purified by ni - nta column and its activity was confirmed
（ 3 ）在大肠杆菌blzi中进行表达，经ni nta柱纯化获得了纯化产物，并证实纯化产物具有与hbsag特异性结合的功能。 |
| 3. | The cytotoxicities of rta and the fusion toxin rta - yqrl were measured by the mtt assay in hela , skov - 3 , and wish cells following fluid - phase endocytosis . [ methods ] 1
因为rta可与f3ga发生特异性结合，我们用bule一sepharose一6b柱对rta和rta一yqrl蛋白分别进行亲和层析纯化。 |
| 4. | ( 2 ) after transiently transfected in cos - 7 cells , secreting expression were detect . jurkat cell lines were construct to express scfvs stably . the activities of the products were confirmed
稳定转染jurkat细胞，经筛选获得稳定表达scfv融合蛋白的细胞系，并证实表达产物具有与hbsag特异性结合的功能。 |
| 5. | The studies provided fundamental data and materials not only for the development of monoclonal antibody ( mab ) , but also for the preparation and development of special diagnosis kits of h5 aiv subtypes
结果表明，该融合蛋白能同h5亚型阳性血清发生特异性结合，并且同h7 、 h9亚型aiv阳性血清没有交叉反应。 |
| 6. | It was testified that the antibody can special immunological recognition the protein gst - cpti and cptl by indirect enzyme linked immunosorbent assay ( elisa ) . the coefficient of correlation is significant and the potency is more than 1 : 800
经间接elisa法检测，抗体能与gst - cpti 、 cpti蛋白特异性结合，其相关系数达到显著水平，效价1 800 。 |
| 7. | In part four , new biological analytical methods based on core - shell bio - nanoparticles have been established and the analysis of biological medicine in suit , in vivo , in real time have been developed under the combination of nanotechnology and biotechnology
苷酸的特异性结合，成功地实现了对互补单链核苷酸片段的高效快速分离和富集，且不改变分离对象的特性。 |
| 8. | 3 . different oligochitosan fragments showed different effect on oligochitosan uptake , suggesting receptor recognition dependent on the structure of oligochitosan 4 . competition studies showed that mannose inhibited oligochitosan internalization , while lps and p - glucan could not . 5 . inhibition of mannose -
2 .壳寡糖在巨噬细胞膜表面有特异性结合的受体( kd为2 . 1x10一sm ) ，可能为一廿露糖受体。 |
| 9. | Doc - 1r gene is an candidate tumor suppressor gene which was discovered by zhangxue . it can interact with cdk2 specifically and restrain cdk2 transportation into the nucleus , which will block the formation of cdk2 and cyclin complex and hinder the process of cell cycle
Doc - 1r基因是张学等发现的一个候选抑癌基因，它可与cdk2特异性结合，抑制cdk2的入核转运，使cdk2不能与cyclin形成复合物，从而阻抑细胞周期的进程。 |
| 10. | After adding culture mediem of stably transfected jurkat cells to hepatocarcinoma cells , the binding specificity of the scfvs with hbsag was further confirmed by observation by fluorescence microscope , indirect immunofluorescence and flow cytometer analysis . prokaryotic expression plasmids ptat - ha - scfvs were successfully constructed
建系细胞培养上清与肝癌细胞作用后，经荧光显微镜观察、间接免疫荧光及流式细胞仪检测进一步确定表达的scfv融合蛋白具有与hbsag特异性结合的活性。 |
