Based upon the comparison of cyto b gene sequences in 15 deer species downloaded from genbank , a universal primer set l15774 / hsf21 was used as positive control of the template quality , at the meantime , two species specific primer sets df / dr and cf / cr were desgined to identify red deer ( cervus elaphus ) , sika deer ( cervus nippori ) and roe deer ( capreolus capreolus ) from other species . a musk deer ( moschus ) specific primer set wf / mr was designed , siberian musk deer ( afoschus moschiferns ) and forest musk desr ( moschus berezovskii ) could be discriminated when restriction endonuclease rsa i was used to cut the pcr products 经过对来自genbank中的15种鹿类动物的cytob基因序列的比较，用通用引物l15774和hsf21作为模板的质量控制，设计了特异性引物df dr和cf cr来鉴定马鹿、梅花鹿、狍；设计了麝类特异性引物mf mr ，用限制性内切酶rsa酶切扩增产物来区分原麝和林麝。
