| 1. | The stable expression yeast was obtained by screening and was induced to ferment by methanol
将ppicgk七质粒电转化gslls酵母；通过筛选获取稳定表达ctla4胞外区蛋白的表达菌。 |
| 2. | Three kinds of parasporal crystals of bipyramidal , cubical and embedded shapes were observed under an electronmicroscope
建立了一个利用电转化技术构建遗传改良工程菌的新模式。 3 |
| 3. | Because of its high conversion efficiency , mature producing techniques and good reliability , solar cell was firstly used in space field
单晶硅太阳能电池因其电转化率高，制造工艺成熟，可靠性好而首先被用于航天领域。 |
| 4. | It was then cloned to the secreted vector - ppic9k and recombined successfully into the chromosome of pichia pastoris host strain - gsl 15 by electroporation
通过电转化作用该基因片段被成功地整合至酵母cs115的染色体上，经过甲醇诱导之后，该基因得到了分泌表达。 |
| 5. | The transformation frequencies of exogenous plasmids in bmb171 were 7 . 5 103 - 8 . 1 108 folds as much as those in ybt - 1463 , and stability of exogenous introduced in 8mb 171 was obviously higher than in ybt - 1463
用pht3101等5种供体质粒电转化bmb171的效率比电转化ybt - 1463的效率显著提高，而转化导入的3种外源质粒在bmb171中的稳定性也明显高于出发菌株ybt - 1463 。 |
| 6. | The very plasmid pbmb0474 was constructed . when the recombinant plasmid pbmb0474 was transferred into crystal negative bt strain bmb171 through electroporation , the expression of the fusion genes about 150kda - 160kda can be detected by sds - page . 4
将pbmb0474电转化到bmb171中得到苏云金芽胞杆菌重组菌， sds - page结果显示重组菌可以表达大小约150kda - 160kda的gfp融合蛋白。 |
| 7. | The artificial antibacterial peptide - ceme gene was designed according to the codon with the highest frequency in pichia pastoris , then the ceme gene was integrated into the chromosome of pichia pastoris strain - gsl 15 by electroporation
我们根据人工设计的新型抗菌肽ceme序列设计ceme基因，重组到酵母胞内表达型的整合载体phild2中，通过电转化作用将ceme基因整合至酵母宿主gsll5染色体上。 |
| 8. | The recombination expression vector , ppic9 - e2 , was linearized by sal i and electroporated into p . pastoris gs115 . recombinant p . pastoris gs115 , designated gs115 - ppic9 - e2 , was identified by pcr and the product of the pcr was analyzed by sequencing before its expression for csfv e2 protein
重组表达载体ppic9 - e2经sal酶切线性化后，电转化整合到毕赤酵母gs115基因组上，经pcr鉴定和pcr产物测序分析，阳性转化子命名为gs115 - ppic9 - e2 。 |
| 9. | After pcr checking and electro - transformation plasmid from c . elegans into dh5a , isolating plasmid from dhsct , digesting them with restriction enzymes and dna sequencing , six cdna fragments , which protein products can interact with rapgap , from cdna library were got
将pcr扩增阳性及或lacz强阳性质粒电转化入dhsa细菌，提取质粒后酶切、测序。发现有6个来源于celegqnscdna文库的dna片段编码的蛋白可以与ra帅ap相互作用，其中两个为rapgap 。 |
| 10. | In this article , the advanced structure of hybrid peptide mae is predicted with software . the fused gene mae - intein - cbd is amplified by pcr with the template of plasmid ptyb2 , and then it is cloned into expression vector plasmid ppic9k . after verified by restriction enzyme analyzing and sequencing , the vector is transferred into the eukaryotic host ( yeast pichia
并以已构建的载体ptyb2 - mae为模板，通过pcr扩增出融合基因mae - imein - cbd ，将其克隆于表达质粒ppic9k中，通过鉴定并测序正确后，电转化真核表达宿主? ?毕赤酵母菌株gs115 ，通过营养缺陷型培养基筛选重组子，再利用g418抗性筛选出整合有多拷贝外源基因的重组子。 |
