| 1. | Changes in proteins within germinating seeds of transgenic wheat with an antisense construct directed gainst the thioredoxin
转反义硫氧还蛋白基因小麦萌发种子中蛋白质的变化 |
| 2. | Because of the cleavage site of enterokinase and cnbr was designed in the middle of thioredoxin and cmiv , the expressed peptides of the mutation of cmiv could be cuted down from the fusion protein by enterokinase or cnbr
由于硫氧还蛋白和抗菌肽之间设计了肠激酶( enterokinase )切割位点和cnbr切割位点，通过对该表达的融合蛋白的切割，可得到目标抗菌肽cmiv突变体多肽分子。 |
| 3. | Homologous analysis in amino acid sequence showed that between thl2 in b . olereacea l . and trx4 in arabidopsis thaliana were 97 % in identity , a little difference to that of 86 % in identity between thl2 in b . napus l . and trx4 in a . thaliana
氨基酸水平上的同源性分析表明甘蓝thl2与油菜thl2和拟南芥中硫氧还蛋白trx4同源性较高。分别达到97和86 ，进化分析也表明thl2和trx4具有较高的亲缘关系。 |
| 4. | We have identified a nucleotide sequence from ests ( expressed sepuence tagged ) acquired fromzap - cdna library of thellungiella salsuginea treated with 200mmol . l - 1 nacl and analyzed its nucleotide sequence characterization , gene organization , and differential expression under salt stress
本实验从200mmol . l ~ ( - 1 ) nacl处理的小盐芥地上部分构建的zap - cdna文库中克隆了编码硫氧还蛋白的基因序列。 |
| 5. | It is the foundation of therapy of hepatitis b . the gene of scfv was cloned into the expression vector pet32a ( + ) and subsequently expressed in escherichia coll . the expressed scfv fused with the 109 aa trx - tag thioredoxin protein was above 30 % total cell protein in the shaking flask cultures
融合硫氧还蛋白的单链抗体在大肠杆菌bl21 ( de3 )中以包涵体的形式得到高效表达，单链抗体表达量达占30的总蛋白以上。 |
| 6. | Both the mature genes of gloshedobin and gussurobin were cloned into the vector pet - 32a ( + ) , strain bl21 ( de3 ) , to study their expression in prokaryotic cell . the gene was expressed under t7 promoter with a fusion protein partner of thx . tag and a 6x his . tag at its n - terminal . having been induced by iptg for 4 hours , the recombinant enzyme was examined in the cytoplasm by sds - page analysis
将大连蛇岛蝮蛇和长白山白眉蝮蛇毒类凝血酶基因分别克隆到大肠杆菌表达载体pet - 32 ( a ) +中，在t7启动子下表达出融合蛋白，融合部分为硫氧还蛋白，位于类凝血酶基因上游，并在其n端带6xhistag标签以利于表达产物的分离纯化，经热激转化至宿主菌bl21 ( de3 )中， iptg诱导斗小时后收获菌体。 |
| 7. | The other is ferredoxin / thioredoxin system located in nonphotosynthetic tissue and cytosol of photosynthetic cell , which includes ferredoxin , ferredoxin dependent thioredoxin reductase , thioredoxin h . this two system constitutes important cellular redox regulatory system and can regulate the redox intercellular environment , metablism and signaling transduction
另一个是nadp ( h ) -硫氧还蛋白系统，该系统是由h -型的硫氧还蛋白、 nadp ( h ) 、和依赖于nadp ( h )的硫氧还蛋白还原酶(一种黄素蛋白)组成。 |
| 8. | The expression of tstrx from different tissue also shows different . these results show that this gene is a stress response gene or plays an important role in slat stress tolerance . aiming to identify the functions of this gene and do some further study , we have cloned the gene into the agrobacterium tumefaciens binary vector prok ii and pcambia 3013
结合实验结果以及用200mmol . l ~ ( - 1 ) nacl处理的小盐芥地上部分构建的zap - cdna文库中得到的编码硫氧还蛋白的cdna序列的相关资料推测： tstrx基因可能在保护盐芥免受因胁迫引起的氧化损伤中起作用。 |
| 9. | Depending on cellular location and function of thioredoxins , there are two thioredoxin systems in high plants , one is nadp / thioredoxin system mainly existing in chloroplast of high plant and eucaryote algae and oxygenic photosynthetic prokaryote , which is composed of nadph , nadph - dependent thioredoxin reductase , thioredoxin - f and thioredoxin - m
在高等植物中，存在两个与硫氧还蛋白密切相关的硫氧还蛋白系统：一个是位于叶绿体中的铁氧还蛋白-硫氧还蛋白系统，该系统中含有两类核基因编码的硫氧还蛋白即f -型trx和m -型trx 。 |
| 10. | In thl1 gene , there are some famililar enzyme digest sites including bamh i , hind , sac i and sal i . the sequence of thl1l from brassica oleracea l . shows 99 % identity to the sequence of thll from brassica napus l . there are some phosphorylation sites and an active site of thoiredoxin h members consisting of cppc in thll protein
甘蓝和油菜thl1基因序列有5个碱基的差异，而氨基酸仅有2个氨基酸的变异，同源性达99 。在thl1蛋白的氨基酸序列中发现了一个硫氧还蛋白家族的活性位点( cppc )和多个磷酸化位点。 |
