| 1. | An improved alkaline lysis adaptive to extracting plasmid dna of bacillus
提取的改良碱裂解法 |
| 2. | Methods : plasmid profile analysis was used
方法：碱裂解法。 |
| 3. | The plasmids of the three bacteria were identified , the results showed m8 had n ' t plasmid , the plasmid of m6 and ml3 were about 20kb
用碱裂解法提取质粒dna ，结果发现： m8没有检测到质粒， m6和m13的质粒大小都为20kb左右。 |
| 4. | The presence of plasmid in bacillus was isolated by improved alkaline lysis . only one plasmid pbl29 was detected in bacillus iicheniformis29
本文采用改良碱裂解法对30株益生芽孢杆菌进行质粒抽提，仅从一株地衣芽孢杆菌b |
| 5. | The plasmid extraction from wild strains needs an appropriate method . first , lysis by alkali was used to extract plasmid from strain pseudomonas xn - 1 . but the expected result had not been got , and a lot of cracked plasmid dna fragments were got only
对于野生菌株的质粒提取，需要选择合适的提取方法，我们首先采用了细菌细胞质粒提取常用的碱裂解法，但是未取得理想的效果，只得到了许多破碎的质粒dna片段。 |
| 6. | Chapter 2 screening of genes related to the ovary development of the mitten crab ( eriocheir japonica sinensis ) by cdna macroarray analysis plasmids were extracted from all the clones in the subtracted cdna libraries with alkali lysis method , then the inserted fragments in plasmids were amplified by pcr
2用cdna微阵列筛选中华绒螯蟹卵巢发育相关基因将差减cdna文库中的所有克隆用碱裂解法提取质粒，然后以提取的质粒为模板，用pcr方法扩增插入片段。 |
| 7. | So some methods suitable to large plasmid extraction , including lysis by sds and a method from a literature , were used to try to extract the large plasmid from the strain cell . the lysis reactions in these two methods are gentle , so the large plasmid cannot be injured in the lysis process , opposite to lysis by alkali . it would be helpful to keep the integrality of the large plasmid during the extraction
因此我们采用了适合于大质粒提取的sds法，和文献中应用于硝基苯降解性质粒的提取方法，来尝试对菌株细胞进行质粒提取，这两种方法裂解反应温和，不会像碱裂解法那样，在裂解过程中损坏质粒，可以实现质粒提取的完整性。 |
| 8. | Methods : the rearranged gene fragment coding tcr y v region of the jurkat cell line was obtained by rt - pcr technique the pcr product was cloned into the eukaryocytic expressive vector pcdnas to construct pcdna3 / tcr y . after confirmed by sequncing . pcdnas / tcr y plasmids were amplified in bacteria extracted by alkaline lysismethod
方法：本文采用rt ? ? pcr的方法扩增jurkatt淋巴瘤细胞特异性重排的tcr可变区基因片段，克隆到真表达载体pcdna _ 3中，经序列测定无误后，碱裂解法大量提取质粒，制备dna疫苗。 |
| 9. | A pair of primers were designed and synthesized based on the published ge gene sequence of prv - rice strain for amplifying ge gene of prv min - a , yielding a 1 . 7kb band . the segment was linked to puc19 plasma dna by means of t4 dna ligase , transformed into e . coli jm109 permissive cells , and incubated on lb fray containg amp , x - gal and iptg . small amount of plasma was extracted by base cleavaging for enzyme digest analysis and pcr , resulting in recombinant plasma puge dna containing prv ge
用t _ 4dna连接酶使ge基因与经bamhi 、 kpni同样双酶切的puc19质粒dna连接；用连接产物转化大肠杆菌jml09感受态细胞，置含amp 、 x - gal和iptg的lb平板上培养12 20小时；挑取白色菌落于选择性培养基扩大培养，碱裂解法小量提取质粒dna ，并进行酶切分析鉴定，结果获得整合有prvge基因的重组质粒pugedna ，并与其它prv分离株进行ge基因序列同源性分析。 |
