| 1. | Bacteriophages are also assayed by a plague assay method based on similar principles .
基于同样原理细菌噬菌体也通过空斑测定法进行检测。 |
| 2. | Plaque experiment indicated that hasnpvgp64 + egfp + can produce infectious virions in sf21 cells
Hasnpvgp64 + egfp +感染sf21细胞的空斑实验，发现在sf21细胞中形成有感染性的病毒粒子。 |
| 3. | 8 schmaljohn c s , and dalrymple j m . hantaviruses , encyclopeolia of virology , vol . 2 , ( edited by r . g . webster and a . granoff ) . p544
7姚小剑，俞永新，安祺.检测流行性出血热病毒滴度和中和抗体的半微量空斑减少中和试验.病毒学报, 1988 ， 4 （ 4 ） 347 - 350 |
| 4. | The immunomodulatory effects of crude product were detected by lymphocyte transformation test ( lit ) and plaque forming cell assay ( pfc )
Kp荚膜糖蛋白粗产品免疫功能检测采用淋巴细胞转化试验( mtt法)和溶血空斑试验，分别测定细胞免疫和体液免疫功能。 |
| 5. | Hear - body - rate test , hemolytic plaque test , hemolysin test and cellular immune function test were studied respectively with mouse by cros - over design in group
摘要以小鼠为试验对象，采用分组交叉对照方式，分别进行小鼠脏体对比试验，溶血空斑试验，溶血素试验，细胞免疫功能试验。 |
| 6. | Results : portulaca extracts improved the devouring percentage and index of macrophage cell and promoted the formation of hemolysin and hemolysis void the transformation of lymphocyte
结果：马齿苋提取物可显著提高小鼠腹腔巨噬细胞的吞噬百分率和吞噬指数；促进溶血素及溶血空斑的形成；促进淋巴细胞的转化。 |
| 7. | Method : the effects of portulaca extracts towards normal rats on the function of macrophage cell in abdominal cavity , the formation of hemolysin and hemolysis void and transformation of hemolysis void were observed
方法：观察马齿苋提取物对小鼠腹腔巨噬细胞吞噬功能、浓血素形成、溶血空斑形成及淋巴细胞转化功能的作用。 |
| 8. | First , sf9 and hzami cells were infected with successfully transfected supernatant . second a transfection - plaque method was used . with both methods , the production of infectious progeny virions was not observed . the results indicated that gp64 would not substitute for the function of ha133
通过其对hzami细胞的转染和上清对sf21细胞的感染及转染-空斑的方法，未检测到在hzami - hasnpv系统中产生有感染性的病毒粒子，表明gp64不能功能性地替代f蛋白ha133 ，并对这一结果进行了讨论。 |
| 9. | Finally , in order to investigate whether these two virus are resistant to complement attack , and whether the resistance were mediated by incorporating cellular complement regulators of cd55 , cd59 into their out membrane , complement lysis assay and plaque reduction assay were carried out respectively to find the lysis or neutralization activity of complement to hiv and eev under the condition of with or without the cd55 and cd59 blocking antibody
将这两种病毒分别暴露于人血清补体后，发现在有cd55 、 cd59中和性抗体存在的情况下，补体溶解hiv病毒的效率明显增加； eev病毒的感染活性在空斑实验中也明显降低，结果与这两种蛋白在病毒表面（ eev ）存在的事实相符，提示病毒表面的cd55 |
| 10. | The wells of elisa plate were coated with pab ( 100ng / l ) against h3n2 , then phage was added to the wells . after incubation , the wells were washed vigorously with tbst to remove nonbinding phage . phage bound to the antibody were eluted with 0 . 2mol / l glycine - hcl ( ph2 . 2 ) for 10 min at room temperature and neutrialized with 2mol / l tris - hcl ( ph9 . 1 )
以抗h3n2流感病毒的多克隆抗体( 100ng l )包被酶标板，加入制备好的肽库，用tbst洗去非特异结合的噬菌体，加0 . 2mol l甘氨酸-盐酸( ph2 . 2 ) ，室温放置10min以洗脱特异结合的噬菌体， 2mol ltris - hcl ( ph9 . 1 )中和后，取2 l噬菌体接种大肠杆菌xl1 - blue菌进行空斑滴定，其余噬菌体扩增后用于下一轮筛选，共重复3轮淘洗。 |
