| 1. | Cytopathic effect , cpe
细胞病变效应 |
| 2. | Relationship between viral load and cytopathic effect during human cytomegalovirus infection
人巨细胞病毒负荷量对细胞病变的影响 |
| 3. | Stem cell disorders
干细胞病变 |
| 4. | Hbeag negative lamivudine - resistant hbvs , which replicate more efficiently and produce higher viral loads , may become directly cytopathic
抗拉米hbv病毒，其复制更加有效并且产生更高的病毒量，并可能直接导致了细胞病变。 |
| 5. | A cell with disease is the cell that has been changed in terms of chi and the small elements . the cure is achieved by correcting the state in which the vibration is confused and unbalanced
细胞病变是因为它的气和小元素改变了，治疗的方法就是改正它混乱而且不平衡的振动状态。 |
| 6. | Bovine viral diarrhea virus were propagated by mdbk cells . after five to six continuously and rapidly passing , the cells emerged evident cytopathic effect . then the infected cells and its solution were harvested
Bvdvnadl株属于致细胞病变型毒株，用mdbk细胞培养增殖bvdv ，经5 - 6次连续传代至出现明显的细胞病变。 |
| 7. | Lycopene is important for men , especially for prostate health . vitamin a and zinc help protect the reproductive organs and together with lycopene can provide a big boost to prostate health
番茄红素对男士健康尤其重要，有效促进前列腺健康，预防前列腺细胞病变；而维他命a和锌也能保护性器官细胞，两者一起补充，男士无后顾之忧。 |
| 8. | The cells were harvested and total e . coli proteins were detected by sds - page to select expressed strains . western - blot analysis of the gel that showed a band of similar size was the major protein immunoreactive with the anti - 6xhis mab
Rpoifn经部分纯化并充分复性后，用细胞病变抑制法测定rpoifn的抗滤泡性口炎病毒( vsv )活性。 |
| 9. | The infected cells were passaged so that the virus could be isolated . cpe were observed from passage 2 to passage 9 postinfection . the cultured virus supernatants were harvested when cpe were observed in 3 / 4 infected cells
接种后的胚肺细胞从第二代到第九代均出现细胞病变，当接毒细胞有3 4出现细胞病变时，收集培养的细胞，制作超薄切片进行电镜观察，发现了病毒样粒子。 |
| 10. | In this experiment hcv structural gene was amplified by polymerase chain reaction ( pcr ) , and was inserted into baculovirus expression vector pfastbacl to construct a recombinant transposing vector pfbl - cee . the plasmid pfb 1 - cee was transformed into dh1 obac competent e . coli cells . high molecular weight dna was prepared from the overnight cultures from the selected e . coli colonies , which was recombinant baculovirus shuttle vector containing hcv structural gene , named bac - cee
本实验用pcr扩增hcv结构区基因，克隆到杆状病毒表达载体pfastbacl中，构建成重组转座载体pfb1 - cee ，转化dh10bac大肠杆菌感受态细胞，筛选阳性菌落，抽提大分子质粒dna ，获得含hcv结构区基因的重组杆状病毒穿梭载体bac - cee ，脂质体介导转染sf9昆虫细胞，出现细胞病变后，收集含有重组杆状病毒颗粒的培养上消，重新感染sf9细胞，收集sf9细胞，进行12 . 5 sds -聚丙烯酰胺凝胶电泳，可见表达的蛋白条带。 |
