The mrna and protein expression were assayed by rt - pcr and sds - page . the results were found that the specific 740bp dna bases of il - 6 was detected by rt - pcr in the recombinant bacteria and a new protein band was found in sds - page with molecular mass of about 49 kda which is consisted of a 23 kda protein deduced from the il - 6 gene sequence and gst ( 26 kda ) 以iptg诱导融合蛋白表达，经rt - pcr检测发现740bp的特异性il - 6条带；通过sds - page分析，在49kd处出现gst ? pil6融合蛋白条带，其表达量占总细菌蛋白量的30 ，证明猪il - 6基因得到了正确转录和表达。
