| 1. | Screening and constructional analysis of extracellular at the ligand binding domain of the vascular endothelial growth factor receptor flt
胞外配体结合域的筛选及其结构分析 |
| 2. | We also predicted the cam binding domain of ked like protein and showed that the cambd lies in its c terminus
对这一蛋白的cam结合域进行了预测，表明ked样蛋白的cam结合域在其c端。 |
| 3. | It is show the mutant consists of a nuclei binding site and dna - binding domain is enough to negative regulation . a report plasmid pcat that we constructed has a cat gene expressed by a cmv early promoter
（ 465 1079氨基酸段）对sv40启动子明显的抑制活性，因为它只包含dna结合域和核结合位点，说明发挥转录抑制功能的关键是ie180的dna结合域。 |
| 4. | To investigate the secondary structure of this gene , we found it has a transmembrane region near the n - terminus , followed by a proline - rich conserved region , and it has a conserved haemachrome binding region about 60 aa away from carboxyl terminus
分析氨基酸序列的二级结构发现基因n -端具有跨膜的疏水序列，其后具有富含脯氨酸保守区，在距离羧基端60个氨基酸处具有高度保守的血红素结合域。 |
| 5. | The gpa1 gene was obtained via pcr amplification and was cloned in the two - hybrid dna binding domain vector pgbkt7 the combinant plasmid was designated as pgbkt7 - ga . - galactosidase assay indicated that got did not have the property of self - activation . after pgbkt7 - ga was transformed to yeast pj69 - 4a , we transformed arabidopsis vegetative tissue two - hybrid cdna library plasmids to yeast pj69 - 4a containing pgbkt7 - ga
通过pcr扩增得到gpa1基因并将其克隆到双杂交dna结合域载体pgbkt7中，得到的重组质粒命名为pgbkt7 - g ， ?半乳糖苷酶活性鉴定表明g不具有自激活特性，将其转化到酵母菌pj69 - 4a中，再以此为受体菌转化拟南芥绿色营养组织cdna文库质粒。 |
| 6. | To neutralize the function of cam , the cam binding domain peptide from bovine calcineurin a subunit was chosen because the pre - experiment demonstrated that bovine calcineurin could bind to calmodulin strongely in an acidic and high calcium concentration condition which is similar to the cell wall environment . in order to increase the binding ability of the chimeric secreted calmodulin binding peptide , the plasmid containing two copies of calmodulin binding domain were constructed
它共有26个氨基酸，具有信号肽的典型特征。为了削弱质外体cam的功能，选择了牛脑钙调神经磷酸酶can ( calcineurin )的a亚基的cam结合域。预实验结果表明，在近似于胞外环境的酸性高钙条件下can与cam有强结合。 |
| 7. | Recently , ldl apheresis has been applied in clinic and achieves a satisactory effect . in this dissertation , the tripeptide , serine - aspartic - glutamic acid ( sde ) , which existes in the cooh - terminal end of the seven repeats in the ligand binding domain of the ldl receptor and plays an important role in identifying ldl , was synthetized and immobilized onto the polyacrylamide ( paam ) beads as a bionic adsorbent for selective removal of ldl from plasma
本论文以丝氨酰-天冬氨酰-谷氨酸( sde )负电性三肽(此三肽广泛存在于ldl受体配体结合域7个重复序列的羧基末端，对ldl受体特异性识别ldl起着重要作用)作为配体固定到聚丙烯酰胺微球载体上制成仿生性ldl亲和吸附剂，考察其对人血浆中ldl及hdl的吸附功效。 |
| 8. | The fusion gene ofgfp : mtn ( mtn is binding domain of microfilament binding protein talin , which can show the microfilament in living cell ) was transferred to arabidopsis thaliana . stable expression of gfp - mtn can visualize the actin cytoskeleton in different types in living cells without affecting cell morphology and function
将gfp : mtn ( mtn是微丝结合蛋白talin的微丝结合域，可以显示活体细胞中微丝的结构)导入拟南芥后，发现表达的融合蛋白能够标记微丝骨架，同时又不影响植物细胞的正常形态和功能。 |
