| 1. | Discussion of the subculture of parent eggs from the perspective of the abnormalities in the grandparent eggs of f
从原原种中的异常现象看母种继代 |
| 2. | Proteins associated with callus proliferation and adventitious bud differentiation of populus euphratica
胡杨愈伤组织继代增殖和器官发生中蛋白分子标记的研究 |
| 3. | Study on influence factors to dormancy during enrichment culture of double petals common sinningia speciosa benth . et hook
重瓣大岩桐继代过程中休眠现象的影响因素研究 |
| 4. | After 4 times selected culture by ppt , 347 resistant calli were obtained . the ppt resistant calli were transferred to ms medium with 2 . 0 mg / l 6 - ba , 0 . 1 mg / l naa for regeneration
0mg l +蔗糖30g l的选择培养基中进行选择培养，每10天继代1次，连续选择4次，共得到347块抗性愈伤组织。 |
| 5. | The optimum concentration of kanamycin was different at different stage of culture , which was 50mg / l at calli induction stage , 100mg / l at embryogenic calli selecting stage , and 50mg / l at plantlet root elongating stage , respectively
把愈伤组织诱导阶段的kan浓度定为somg / l ;愈伤组织继代的kan浓度定为100m叭;小植株长根的培养基的kan浓度定为som叭。 |
| 6. | The main results are as follows : ( 1 ) plant regeneration system of tall fescue embryogenic calli was established according to studies on impacts of manifold factors on callus induction from mature seeds and subculture production and differentiation of embryogenic calli
主要研究结果如下： ( 1 )通过对成熟种子愈伤组织诱导、胚性愈伤组织继代发生和分化的多种影响因素的研究，建立了高羊茅胚性愈伤组织植株再生体系。 |
| 7. | 2 . the resistence of transformators were selected by g418 after co - culture with agrobactrium tumeflien , which was 40 mg / l in subcultured medium and 50mg / l in differeniated medium . in the process of killing agrobactrium , cef , amp or carb were also useful and the time of inhibition was long too
经与农杆菌共培养后转化子的鉴定选用g418 ，在继代筛选培养基中添加40mg l ，在分化筛选培养基中添加50mg l ；在除菌过程中，头孢噻肟钠、羧苄青霉素、氨苄青霉素抑菌效果较好，抑菌时间较长。 |
| 8. | There are also different in inducing cluster buds from callus of clone gingkgo and its other tissues and organs , some callus can induce cluster buds , but others can not . differentiation rate from the callus of cotyledon can be up to 36 % and the number of cluster buds is positive related to the times of subculturing
但是不同器官诱导的愈伤组织对于丛生芽的诱导也是不同的，子叶诱导的愈伤组织的分化率较高，有36左右，并且继代次数的增加也能够增加分化的几率 |
| 9. | Pre - differentiation and proper partial desiccation of calli before transferred to regeneration medium was found to apparently improve the frequency and quality of plant differentiation . with our optimized culture condition and treatment style , induction frequency of pei ' ai64s and 9311 can be reached 62 . 45 % and 85 . 30 % respectively and after 3 months of subculture calli can remain high - quality embryogenic state , when high - quality embryogenic calli after two times of subculture were used as acceptor , callus differentiation frequency can arrive at 85 . 5 % and 87 . 7 % respectively
采用我们优化后的培养条件与处理方式，培矮64s和9311愈伤组织的诱导频率分别可达到62 . 45和85 . 30 ；继代培养时间达三个月左右仍能保持较好的胚性生长状态；对于继代两次胚性生长状态良好的愈伤组织分化频率分别可达到85 . 5和87 . 7 。 |
| 10. | Many researchers have conducted experimellts about it , but not succeeded . culture media were compared to find the best medium of gingkgo culture for controlling callus browning by different sugars , antioxidants and sorbents . the result showed that the medium with ms + zt 1 . 0mg / l + naa 1 . 0mg / l + sucrose 50g / l + agar 8g / l + ac 2g / l was the best medium , at l5 days subculturing intervals
银杏组织培养过程中，尤其是在愈伤组织的继代培养中，褐变现象特别严重，曾有不少的人做过这方面的研究，但都没有成功，而本研究通过对不同糖类物质、抗氧化剂、吸附剂以及不同的培养基对褐变的影响和控制效果，探索出有效控制褐化现象发生的最佳培养条件，试验结果表明： ms + zt1 . 0mg / l + naa1 . 0mg / l +蔗糖50g / l +琼脂8g / l + ac2g / l培养基上的继代效果最好，继代时间最好在15d左右。 |
