| 1. | The role of a mid - tier soa cache architecture 中间层soa缓冲体系结构的作用 |
| 2. | There were 5 . 9 tip residues in cml . the purified cml was modified with n - bromosuccinimide ( nbs ) in hac - naac buffer containing 8mol / l urea or not 在含8mol l脲和不含脲的缓冲体系中,用n -溴代丁二酰亚胺( nbs )修饰cml的色氨酸残基。 |
| 3. | Using ki , cscl and acr to probe the micro - environments of trp residues in dpl , the trp residue fluorescence of dpl was not quenched by ki and cscl , but 100 % quenched by acr 在含脲的体系中可修饰36个色氨酸残基;在不含脲的缓冲体系中可修饰12个色氨酸残基。 |
| 4. | Wang shun , lin juanjuan et al . , kinetics and mechanism of the reaction between thiourea and iodate in unbuffered medium , science in china , ser . b , 2004 , 47 ( 6 ) , 480 王舜,高庆宇,王新红,林娟娟,亚氯酸盐-硫代硫酸盐非缓冲体系的复杂动力学,物理化学学报, 2003 , 19 ( 8 ) , 762 |
| 5. | The trials of nr / r single - dimensional sds - page and nr / r two - dimensional sds - page proved that cml had no disulfide bond . edta inhibited the agglutinate activity of cml and a small part of cml recovered its agglutinate activity after removing the edta 在ph7 . 0 、 ph5 . 0的缓冲体系中,用edta去除cml分子中金属离子的实验表明edta对cml的凝血活性具有抑制作用。 |
| 6. | The result indicated 1 . 8 tip residues were modified in the buffer without urea and they were not essential groups to the agglutinate activity of cml . in the buffer with urea , 5 . 0 tip residues were modified and 2 . 8 of them were essential groups to the agglutinate activity of cml 含脲体系中可修饰5 . 0个色氨酸残基,其中有2 . 8个为cml活性所需;不含脲缓冲体系中可修饰1 . 8个色氨酸残基,都不是cml活性所必需。 |
| 7. | Based on investigating numerous references in literature both at home and abroad , collection of statistical data and field survey , this paper conducts a primary study on pollution control of agricultural non - point source by means of riparian buffer in shanghai for the first time 本研究选取了上海松江泗泾镇古楼村为示范区,从2002年4月到2003年12月,在上海首次进行了农业面源污染控制的滨岸缓冲体系的初步研究。 |
| 8. | Cml ' s pi is 5 . 7 . the purified cml modified by n - ethymaleimide in the physiological saline containing 8mol / l urea or not did n ' t lose its agglutinate activity . about 3 . 1 sulfhydryl groups in cml were modified in 8mol / l urea and 3 . 2 sulfhydryl groups in cml were modified in the physiological saline without urea 在含8mol l脲和不含脲的缓冲体系中,用n -乙基顺丁烯二酰亚胺( nem )修饰cml的巯基,计算每个cml分子中被修饰的巯基数目分别为3 . 1和3 . 2个,修饰后cml的凝血活性无明显变化。 |
| 9. | The results indicated that the synthetic mviia was reduced and oxidized in a buffer containing acetamide / guanidine hydrochloride by air oxidization in the room temperature and obtained a relatively well oxidization result , the synthetic mviia showed 100 % of physiological activity of native mviia , but obtained 1 % product of synthetic crude peptide 结果表明,合成的mviia在乙酸胺/盐酸胍缓冲体系,室温下采用空气氧化法时获得了较好的复性结果,合成复性的mviia几乎具有天然mviia的100的生物学活性,但产率只有1左右。 |