The gene of amoa in ammonia - oxidizing encodes the active - site polypeptide of ammonia monooxygenase which catalyzes the oxidation of ammonia to hydroxylamine . we designed a pair of primers special for the amoa gene by comparing the known amoa gene sequences and used pcr to amplify the amoa gene fragments Amoa基因是编码氨单加氧酶活性多肽位点基因，我们通过引物筛选合成了对氨氧化细菌amoa基因特异结合的引物序列，利用pcr技术对活性污泥中的amoa基因片段进行特异扩增，得到的dna片段大约为490bp 。
