| 1. | In the research ts containing antigenic sites b and c was expressed with high efficiency
本研究高效表达了tgev含有b和c抗原位点的多肽片段( ts ) 。 |
| 2. | Proteins were identified with peptide mass fingerprinting using matrix - assisted laser desorption ionization time of flight mass spectrometry ( maldi - tof ms ) after tryptic in - gel digestion
差异蛋白经胰蛋白酶酶切后产生肽片段，再利用基质辅助激光解吸电离飞行时间质谱得到肽指纹图谱来鉴定。 |
| 3. | The maldi peaks corresponding to the c - terminal peptide fragments of proteins were specifically enhanced , discriminating against those from internal peptides that were not tagged with a positive charge
与那些没有正电荷标记的中间肽段的maldi谱图峰明显不同的是，蛋白质c端肽片段的maldi谱图峰明显增强。 |
| 4. | Angiostatin ( as ) is a kind of polypeptide identified as a product of plasminogen cleavaged by protease . it blocks neovascularization by directly inhibiting endothelial cell proliferation and migration
血管抑素( angiostatin ， as )是血浆纤溶酶原( plasminogen ， plg )经蛋白水解酶降解生成的多肽片段，是直接作用于血管内皮细胞的血管生成抑制剂。 |
| 5. | C57bl / 6 ( h - 2b background ) mice were subcutaneously immunized with selected peptides in ifa ( incomplete freund ' s adjuvant ) respectively . 8 days later after immunization , spleens were removed to prepare splenocytes suspension
将这些肽片段分别与不完全弗氏佐剂制备成乳浊液，皮下注射免疫c57bl / 6小鼠， 8天后，处死小鼠，取出脾脏制备脾细胞悬液。 |
| 6. | These findings of the specific ctls epitopes in irbp and pedf may lead to improved understanding of the pathogenesis of uveitis . our study also provides a strategy to identify specific ctls epitopes within tumour antigen , which is helpful to make tumour vaccine for the patients
本研究也为寻找肿瘤抗原中具有诱导特异性ctls能力的肽片段提供了一种策略，为制备肽片段肿瘤疫苗提供了理论基础。 |
| 7. | Based on the " self - migrating and surface - segregation " behavior , the amphiphile segregation surface was therefore constructed on hydrophobic pdl - la matrix , which containing the structure of peo spacer combining cell adhesion ligands to mimic the extracellular matrix ( ecm )
基于两亲共聚物在疏水高聚物材料内的自迁移和表面富集行为，在聚乳酸材料上构建了以peo桥联氨基酸或整合素配体多肽片段的类细胞外基质表面。 |
| 8. | While there are some difference in amino acid with other group ( except tw 95 ) , the amino acid in 65 - 75 of hn of ndv group are conserved , while he in 81 is conserved . based on epitope prediction , five pairs primers are designed with enzyme sites . five segments of f1 , f2 , f3 , f4 and hn1 coding epitope was amplified and cloned to prokaryotic expression vector , respectively
在表位预测的基础上，将临近的表位合并，设计引物并引入酶切位点，以fa 、 fb 、 hna为模板扩增含抗原表位的多肽片段f1 、 f2 、 f3 、 f4 、 hn1 ，克隆到原核表达载体ppro ~ ( ex ) ht上，成功地构建了五个表达载体。 |
| 9. | In he - 3 and de - 3 , 16 protein spots that were absolutely different ( only expressed in diploid embryos but not in haploid embryos or vice versa ) and 16 protein spots that were up - and down - r gulated were identified unambiguously . in he - 2 and de - 2 , 20 protein spots that were absolutely different ( only expressed in diploid embryos but not in haploid embryos or vice versa ) and 5 protein spots that were up - and down - regulated were identified unambiguously , some of these different proteins are correlative with eyes development , nerve development , development regulation , cell differentiation and maintenance , arthromere formation and signal transduction . it shown that gene expression have significant difference between diploid and haploid during embryos developing
分别选取其中70个分辨好的差异蛋白质点进行原位胰蛋白酶酶解，酶解的肽片段用基质辅助激光解析电离分行时间质谱( maldi - tofms )得到肽质指纹图谱，再在网上peptident软件中搜索swiss - prot和trembl数据库，初步鉴定了he - 3和de - 3上32个差异蛋白质点，其中包括16个表达量上有差异的蛋白质点( p 0 . 05 )和16个有无表达的差异蛋白质点，并且初步鉴定了he - 2和de - 2上25个差异蛋白质点，其中包括5个表达量上有差异的蛋白质点( p 0 . 05 )和20个有无表达的差异蛋白质点。 |
| 10. | The study of in - situ construction of cytocompatible surface on pdl - la matrix via amphiphile - amino acid ( rgd ) hybrid self - segregation - the amphiphilic diblock copolymer , poly ( dl - lactide ) - poly ( ethylene oxide ) ( pla - peo ) copolymer , containing hydrophobic pla block and hydrophilic peo block was synthesized via coupling method in this dissertation . cell - adhesion - promoting amino acids and integrin receptor peptide rgd were then immobilized at the end of peo chain of pla - peo copolymer via hydroxyl group activation technique . the solvent blending and casting method was then used to obtain the amphiphile modified pdl - la membranes
两亲共聚物-氨基酸( rgd )杂化体原位自修饰构建聚乳酸细胞相容性表面的研究一本论文首先设计并合成了一类含疏水聚乳酸( pla )链段和亲水聚氧乙烯( peo )链段的两亲嵌段共聚物材料( pla - peo ) ，利用peo链端的活性官能团羟基固定了促细胞粘附的氨基酸及整合素配体多肽片段rgd 。 |
