| 1. | Cloning and sequencing of human cbfa1 cdna fragment from osteosarcoma cell
胞外区片段的克隆和序列分析 |
| 2. | Cloning of extracellular domain fragment of human light gene and expression of fusion protein
胞外区基因的克隆及融合蛋白的表达 |
| 3. | The stable expression yeast was obtained by screening and was induced to ferment by methanol
将ppicgk七质粒电转化gslls酵母；通过筛选获取稳定表达ctla4胞外区蛋白的表达菌。 |
| 4. | The expression of target protein was identified with dot - elisa , sds - page and western blotting
Elisa 、 sda page 、 western blot等方法在蛋白水平对ctla4胞外区蛋白的表达进行了鉴定。 |
| 5. | Meanwhile , because the fragment of ctla4 extracellular domain is only 400bp , its expression will be more stable than that of ctlaig fusion protein ( 1 . 2kb )
同时，由于ctla4胞外区片段大小仅有400hp ，较ctla4ig融合蛋白zkb ）在表达菌中的表达将更为稳定。 |
| 6. | The present investigation is favorable for screening the new generation of anti - tumor peptides . [ objective ] ( 1 ) to clone and express fasl - ecd in e . coli
[目的] ( 1 )分析人黑色素瘤细胞( a375 )中fasl基因的表达，克隆人fasl胞外区的编码基因，并在大肠杆菌bl21中进行表达。 |
| 7. | Inspection for the biological activities of recombination protein of ? ctla4 extracellular domain in vitro : we investigated the role of ctla4e in t cell proliferation of mlr with 3h - tdr incorporation
重组人ctla4胞外区蛋白体内生物学活性研究： l ）观察ctla4胞外区蛋白在小鼠变应性鼻炎中的作用。 |
| 8. | Thereof the ten - peptide is chemically synthesized , and its apoptosis - inducing activity on tumor cells bearing fas is investigated in parallel with recombinant excellular domain of fasl ( fasl - ecd )
化学合成了十肽结构，与重组fasl - ecd ( fasl胞外区)平行对照，研究了其对培养的肿瘤细胞的相互作用。 |
| 9. | After ultrafiltration and rough separation with ammonium sulfate and anion - exchange chromatography , the recombination protein of ctla4 extracelluar domain was rectified from the ferment supernatants
表达菌甲醇诱导发酵；发酵上清液经超滤、硫酸胺粗分级分离以及阴离于交换层析，纯化出ctla4胞外区蛋白。 |
| 10. | Hence , yeast gs115 was used to express the target protein in this study . objection : to obtain the biological activated recombianated protein of huaman ctla4 extracellular domain ( ctla4e ) , and to explore its effects on allergic diseases and transplant rejection
目的：获取具有生物学活性的重组人ctla4胞外区蛋白（ ctla4e ） ，探讨其在变应性疾病和器官移植排斥反应防治中的作用和机理。 |
