| 1. | A study on the enzymic hydrolysis of glu thalli protein in the production of monosodium glutamate
谷氨酸菌体蛋白的酶解试验研究 |
| 2. | Accumulation of single cell protein by purple non - sulfur photosynthetic bacteria in starch waste water treatment
光合细菌法降解淀粉废水积累菌体蛋白的研究 |
| 3. | We can draw a conclusion that the mixture can promote psb reproduction and protein content
实验结果表明，气态混合物能够促进光合细菌的生长，菌体蛋白含量明显增加。 |
| 4. | The highest antiviral litre of rpoifn - a was l l08iu / mi . the rpoifn - a protected pk - 15 cells against virulent hog cholera virus ( hcv ) infection in vitro
Sds - page分析表明，构建的重组表达质粒pqe30 poifn在大肠杆菌jm109中表达的rpoifn占菌体蛋白总量的20 26 。 |
| 5. | And the expression product was an membrane - toxic immunotoxin . the activity determination in vitro indicated the product had significant killing effect to a43i cell of squamous epithelium carcin
破壁后提取ecoil中的目的产物会由于菌体蛋白的大量搀杂而使提纯步骤繁琐，降低得率。 |
| 6. | The inclusion bodies of recombinant protein were purified with washing buffer consisting of various urea ( 2mol / l and 4mol / l ) for several times , and then dissolve the fusion protein in the denature buffer using 8mol / l urea as denaturant
用含有2mol l和4mol l尿素的包涵体洗涤液洗涤包涵体，在37条件下，洗去了大部分菌体蛋白及其它核酸物质。用8mol l尿素作为变性剂溶解包涵体，包涵体在8mol l尿素中的溶解性非常好。 |
| 7. | In this study , we designed a pair of primers based on the sequence of the upstream and downstream of chicken il - 2 gene . about 600 bp chicken il - 2 cdna fragment was cloned from cona - stimulated chicken splenocytes by reverse transcription - polymerase chain reaction ( rt - pcr ) and was subcloned into puc18 vector . recombinant clone was demonstrated by restriction enzyme digestion and dna sequencing . next , we construct recombinant plasmid pproex ? t - il - 2 . the cdna of chicken il - 2 gene was subcloned into bamh i / hind iii sites of vector . the recombinant plasmid pproex ? t - il - 2 was transformed into e . coli dh5a and the bacteria was induced with iptg . it was demonstrated by sds - page and western blot that a 18kda protein which was equal to chicken il - 2 protein in molecular weight was expressed in e . coli dh5a . the expression level was up to 30 % of the total bacterial proteins . the purified protein was used to prepare the antibody against chicken il - 2 protein
经酶切鉴定及dna序列测定，该基因为鸡il - 2基因，其序列与sundick等报道的完全一致。在此基础上，我们把鸡il - 2基因亚克隆到大肠杆菌原核表达载体pproex ~ ( tm ) ht中，构建重组表达质粒并进行确证性序列测定，重组质粒测序结果表明将编码鸡il - 2成熟蛋白的基因正确地插入到原核表达载体pproex ~ ( tm ) ht的目的位点。重组质粒转化受体菌dh5后用iptg于37进行诱导培养， sds - page和westernblot分析显示，表达的鸡il - 2融合蛋白分子量约为18kda ，表达的融合蛋白经薄层扫描发现目的蛋白表达量约占菌体蛋白的30 。 |
| 8. | Sds - page analysis suggested that the bacteria containing the recombinant plasmid pet - 32a ( + ) - igf - i produced the fusion protein of 30kda as it was induced by iptg . consisting 10 % of the total bacterial proteins , and the pet - 30a ( + ) - igf - ii produced the fusion protein of 14kda , which consisting 35 % of total bacterial proteins . 5
Sds - page分析表明，重组质粒pet - 32a ( + ) - igf -在iptg诱导下表达分子量约30kda的融合蛋白，但其表达量不高，约为菌体总蛋白的10左右；重组质粒pet - 30a ( + ) - igf -在iptg诱导下表达分子量约14kda的重组蛋白，融合蛋白表达量约占菌体蛋白总量的35 。 |
| 9. | The reults of sds - page and western blot dermonstrated that the insoulable component of the induced e . coli culture contained protein 3a . the results of the study indicated that protein 3a existed in the form of inclusion body . the content of the expressed protein in the induced bacteria protein was 29 . 2 % and 22 . 1 % respectively
Sds - page和westernbolt结果证实，大肠杆菌菌体不可溶性蛋白中富含3a蛋白，且此融合蛋白的分子量符合预期设计，说明3a蛋白在表达产物中以包涵体的形式存在，所表达的蛋白含量分别占菌体蛋白的29 . 2和22 . 1 。 |
| 10. | After bacterium cells were treated by formaldehyde , surface proteins of gram - negative bacterium e . coli k12 were eluted little , but it had no influence on adsorption of snake venom proteins to bacterium cells . treatment by formaldehyde had little influence on snake venom absorption to and deabsorption from gram - positive bacterium streptococcus mutans , but absorbance of interaction protein with molecular weight of 47kda only adsorbed by streptococcus aureus was greatly decreased
结果表明，灭活后的革兰氏阴性菌( e . colik12 )再与蛇毒相互作用时，洗脱下来的蛋白中菌体蛋白含量明显减少，但不影响对作用蛋白的吸附；甲醛灭活对革兰氏阳性菌( s . mutans和s . aureus )影响不大，只有s . aureus吸附蛋白中47kda的蛋白吸附量减少，不会影响作用蛋白中其它蛋白的吸附。 |
