| 1. | Studies on the proteolytic activity of various lactic acid bacteria fermented milk
乳酸菌蛋白水解力的测定及研究 |
| 2. | The sds - page analysis revealed that the trka extracellular domains proteins were highly expressed and accumulated up to above 30 % of the total bacterial proteins after iptg induction
称a膜外域结构域重组蛋白表达量均占全菌蛋白的30 %以上。 |
| 3. | By means of pcr , the signal peptide dna sequence was deleted . for study of the limited factors in expression of pro - urokinase , the pro - uk gene was divided into three fragments , and they were expressed in
最后用同样的方法提高全长人尿激酶原cdna在大肠杆菌中的表达量，使其表达量达到全菌蛋白的5 % 。 |
| 4. | Tuberculin is one kind fosters filtrate to be made by n / med tuberculosis bacterium , contain the albumen of n / med tuberculosis bacterium , biological product that does not contain substance of n / med tuberculosis bacterium only
结核菌素是一种由结核菌培养滤液制成、只含结核菌蛋白、不含结核菌体的生物制品。 |
| 5. | The engineered strain ghd - yz26 / e . coli bl21 ( de3 ) was incubated and induced at 30 for 8 hrs . the results show that the target fusion protein is soluble and active , which is about 15 % compared with the total proteins in cells , and the enzymatic activity reaches 5 u / ml that is about 8 folds compared with the activity of strain yz - 26
Colibl21 ( de3 )中30诱导培养8hrs ，可获得可溶性表达，融合蛋白的表达量约占总菌蛋白的15 ，其酶活力为5u ml ，约是原始菌株的d -海因酶表观活性的8倍。 |
| 6. | With the procedures , the overall recovery of enzymatic activity reached 20 % and the specific activity for substrate hydantoin was about 4 u / mg protein . the purification factor was about 4 . 7 folds with the purity about more than 95 % as estimated by sds - page analysis . d - hydantoinase gene from strain ss - ori was cloned to five different vectors to be five recombinant plasmids and in turn to transfer into five different e . coli strains , respectively
对其中产酶活性最高的一工程菌pexsec一hdt /云coljblz ] ( de3 )海因酶的表达条件进行了研究，目的蛋白的表达量约占总菌蛋白的20 % ，其酶活力为0 . 92u / ml ，约是原始菌株的d一海因酶表观活性的4 . 6倍。 |
| 7. | Ptxb1 - hng and ptxb1 - m - insulin are expressed in e . coli successfully . after sds - page and densitometric scan analysis , the results show that the expression level of hng fusion protein is above 40 % and m - insulin fusion protein above 50 % of total bacterial proteins . western - blot result demonstrated m - insulin fusion protein had specific reaction with mouse anti human insulin antibody ( igg )
Pbv220 ? hng在大肠杆菌中未检测到表达，后两个克隆在大肠杆菌bl21 ( de3 )中获得高效表达， hng及m - insulin融合蛋白表达量分别占全菌蛋白的40及50左右；经western - blot鉴定m - insulin融合蛋白可以与小鼠抗人胰岛素单克隆抗体( igg )发生抗原抗体结合反应。 |
| 8. | The engineered strain ehd - yz26 / e . coli bl21 ( de3 ) was incubated at 30 for 10 hrs without adding any inducers . the results show that the target product is soluble and active , which account for about 20 % compared with the total proteins in cells , and the enzymatic activity reaches 13 u / ml that is about 13 folds compared with the activity of strain yz - 26
Colibl21 ( de3 )中30生长10hrs ，在不需添加任何诱导剂的情况下可获得可溶性表达，目的蛋白的表达量约占总菌蛋白的20 ，其酶活力为13u ml ，约是原始菌株的d -海因酶表观活性的13倍。 |
| 9. | These proteins are ubiquitous in the plant kingdom where they form a multiple genetic family . they have been suggested to be involved in dif ferent aspects of plant physiology and cell biology through their ability to bind and / or carry lipophilic compounds , including the formation of cutin by transporting the hydrophobic cutin monomers to the apoplast and the defence of plants against pathogens as antimicrobial agents and in flowering
此外nsltp在植物体内广泛存在并由一个基因家族编码，由于它能结合和转运脂类物质，因此推测它可能参与植物体内许多不同的生理过程，如转运蜡质的角质单体到表皮细胞外侧，作为抑菌蛋白抵抗病原菌的入侵以及花粉和柱头的识别等。 |
